Overview

  • Product name

  • Description

    Rabbit polyclonal to MED19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human MED19 (internal sequence). The exact sequence is proprietary.
    Database link: A0JLT2

  • Positive control

    • WB: Jurkat, Raji and NCI-H929 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 19 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • LCMR1 antibody
    • Lung cancer metastasis related protein 1 antibody
    • Lung cancer metastasis-related protein 1 antibody
    • med19 antibody
    • MED19_HUMAN antibody
    • Mediator complex subunit 19 antibody
    • Mediator of RNA polymerase II transcription subunit 19 antibody
    see all

Images

  • All lanes : Anti-MED19 antibody (ab229325) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

References

ab229325 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229325.
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