Overview

  • Product name

  • Description

    Rabbit polyclonal to MED25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human MED25 aa 621-674.
    Sequence:

    PPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHL QPPG


    Database link: Q71SY5

  • Positive control

    • WB: RT4 and U-251 MG cell lysates. ICC/IF: A549 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 78 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • Tissue specificity

    Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
  • Involvement in disease

    Defects in MED25 are the cause of Charcot-Marie-Tooth disease type 2B2 (CMT2B2) [MIM:605589]. It is a recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced.
  • Sequence similarities

    Belongs to the Mediator complex subunit 25 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACID1 antibody
    • Activator interaction domain 1 protein antibody
    • Activator interaction domain-containing protein 1 antibody
    • Activator-recruited cofactor 92 kDa component antibody
    • ARC/mediator transcriptional coactivator subunit antibody
    • ARC/mediator transcriptional coactivator subunit ARC92 antibody
    • ARC92 antibody
    • BVSYS antibody
    • CMT2B2 antibody
    • DKFZp434K0512 antibody
    • FLJ10193 antibody
    • med25 antibody
    • MED25 protein antibody
    • MED25_HUMAN antibody
    • Mediator complex subunit 25 antibody
    • Mediator of RNA polymerase II transcription subunit 25 antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (S cerevisiae) antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) antibody
    • MGC70671 antibody
    • p78 antibody
    • prostate-derived protein 78 kDa antibody
    • PTOV2 antibody
    • TCBAP0758 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for MED25 (green) using ab221741 at 4 µg/ml in ICC/IF. 

  • All lanes : Anti-MED25 antibody (ab221741) at 1/250 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 (human brain glioma cell line) cell lysate

    Predicted band size: 78 kDa

References

ab221741 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221741.
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