Overview

  • Product name

    Anti-MED26 antibody [2216C1a]
    See all MED26 primary antibodies
  • Description

    Mouse monoclonal [2216C1a] to MED26
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blot, ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MED26.

  • Positive control

    • Recombinant MED26 fragment.
  • General notes

     This product was previously labelled as CRSP7

     

Properties

Applications

Our Abpromise guarantee covers the use of ab50619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).
Dot blot Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 24466310

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 26 family.
    Contains 1 TFIIS N-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator recruited cofactor 70 kDa component antibody
    • Activator-recruited cofactor 70 kDa component antibody
    • ARC 70 antibody
    • ARC70 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 7 antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7 (70kD) antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa antibody
    • CRSP 7 antibody
    • CRSP complex subunit 7 antibody
    • CRSP70 antibody
    • MED 26 antibody
    • Med26 antibody
    • MED26_HUMAN antibody
    • Mediator complex subunit 26 antibody
    • Mediator of RNA polymerase II transcription subunit 26 antibody
    • Transcriptional coactivator CRSP70 antibody
    see all

Images

  • Anti-MED26 antibody [2216C1a] (ab50619) + immunized recombinant MED26 fragment

    Predicted band size: 65 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Note: actual size differs from predicted size as this blot was loaded with MED26 fragment.

References

This product has been referenced in:

  • Sato S  et al. Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme. J Biol Chem 291:26886-26898 (2016). Read more (PubMed: 27821593) »
  • Fowler T  et al. Regulation of MYC expression and differential JQ1 sensitivity in cancer cells. PLoS One 9:e87003 (2014). ChIP ; Human . Read more (PubMed: 24466310) »
See all 2 Publications for this product

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