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  1. Link

    med26-antibody-ab214367.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-MED26 antibody (ab214367)

  • Datasheet
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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED26 antibody (ab214367)

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Primary

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Secondary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-MED26 antibody
    See all MED26 primary antibodies
  • Description

    Rabbit polyclonal to MED26
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MED26 aa 30-65 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    AVLEVISSLEKYPITKEALEETRLGKLINDVRKKTK


    Database link: O95402

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human gastric carcinoma tissue.
  • General notes

     This product was previously labelled as CRSP7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human MED26 protein (ab160489)
  • Related Products

    • Recombinant Human MED26 protein (ab160489)

Applications

Our Abpromise guarantee covers the use of ab214367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 26 family.
    Contains 1 TFIIS N-terminal domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O95402 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9441 Human
    • Entrez Gene: 70625 Mouse
    • Entrez Gene: 306328 Rat
    • Omim: 605043 Human
    • SwissProt: O95402 Human
    • SwissProt: Q7TN02 Mouse
    • SwissProt: Q8BUP9 Mouse
    • Unigene: 356467 Human
    • Unigene: 729178 Human
    • Unigene: 235885 Mouse
    • Unigene: 440960 Mouse
    see all
  • Alternative names

    • Activator recruited cofactor 70 kDa component antibody
    • Activator-recruited cofactor 70 kDa component antibody
    • ARC 70 antibody
    • ARC70 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 7 antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7 (70kD) antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa antibody
    • CRSP 7 antibody
    • CRSP complex subunit 7 antibody
    • CRSP70 antibody
    • MED 26 antibody
    • Med26 antibody
    • MED26_HUMAN antibody
    • Mediator complex subunit 26 antibody
    • Mediator of RNA polymerase II transcription subunit 26 antibody
    • Transcriptional coactivator CRSP70 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED26 antibody (ab214367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED26 antibody (ab214367)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma labeling MED26 with ab214367 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Protocols

  • Immunohistochemistry protocols

Datasheets and documents

    • Datasheet
  • References

    ab214367 has not yet been referenced specifically in any publications.

    Publishing research using ab214367? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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