Anti-MED28/EG1 antibody (ab73294)
Key features and details
- Rabbit polyclonal to MED28/EG1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED28/EG1 antibody -
Description
Rabbit polyclonal to MED28/EG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. -
Tissue specificity
Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. -
Sequence similarities
Belongs to the Mediator complex subunit 28 family. -
Cellular localization
Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated. - Information by UniProt
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Database links
- Entrez Gene: 80306 Human
- Omim: 610311 Human
- SwissProt: Q9H204 Human
- Unigene: 434075 Human
- Unigene: 644788 Human
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Alternative names
- EG1 antibody
- Endothelial-derived protein 1 antibody
- FKSG20 antibody
see all
Images
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All lanes : Anti-MED28/EG1 antibody (ab73294) at 1 µg/ml
Lane 1 : human brain tissue lysate
Lane 2 : human brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
ab73294 at 2.5µg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab73294 has not yet been referenced specifically in any publications.