Overview

  • Product name

  • Description

    Rabbit polyclonal to MED28/EG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MED28/EG1 (internal sequence).

  • Positive control

    • Human brain tissue lysate.
  • General notes

     This product was previously labelled as MED28

     

Properties

Applications

Our Abpromise guarantee covers the use of ab73294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
  • Tissue specificity

    Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.
  • Sequence similarities

    Belongs to the Mediator complex subunit 28 family.
  • Cellular localization

    Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
  • Information by UniProt
  • Database links

  • Alternative names

    • EG1 antibody
    • Endothelial-derived protein 1 antibody
    • FKSG20 antibody
    • Magicin antibody
    • med28 antibody
    • MED28_HUMAN antibody
    • Mediator complex subunit 28 antibody
    • Mediator of RNA polymerase II transcription subunit 28 antibody
    • Merlin and Grb2-interacting cytoskeletal protein antibody
    • Tumor angiogenesis marker EG-1 antibody
    see all

Images

  • All lanes : Anti-MED28/EG1 antibody (ab73294) at 1 µg/ml

    Lane 1 : human brain tissue lysate
    Lane 2 : human brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • ab73294 at 2.5µg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • ICC/IF image of ab73294 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab73294 has not yet been referenced specifically in any publications.

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