Key features and details
- Rabbit polyclonal to MED28/EG1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MED28/EG1 antibody
See all MED28/EG1 primary antibodies
DescriptionRabbit polyclonal to MED28/EG1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human MED28/EG1 (internal sequence).
This product was previously labelled as MED28
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
Tissue specificityWidely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.
Sequence similaritiesBelongs to the Mediator complex subunit 28 family.
Cellular localizationNucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
- Information by UniProt
- EG1 antibody
- Endothelial-derived protein 1 antibody
- FKSG20 antibody
All lanes : Anti-MED28/EG1 antibody (ab73294) at 1 µg/ml
Lane 1 : human brain tissue lysate
Lane 2 : human brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
ab73294 at 2.5µg/ml staining MED28/EG1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ab73294 has not yet been referenced specifically in any publications.