Anti-MED3 antibody (ab172363)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MED3 antibody
See all MED3 primary antibodies -
Description
Mouse polyclonal to MED3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Full length protein corresponding to Human MED3 aa 1-311. (NP_004260.2)
Sequence:MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREK AFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDK TPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKA QPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKV IVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTAL LHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFR TLEAFHDTCRQ
Database link: Q6P2C8 -
Positive control
- MED3 transfected 293T cell lysate
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General notes
This product was previously labelled as CRSP8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab172363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Sequence similarities
Belongs to the Mediator complex subunit 27 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 525389 Cow
- Entrez Gene: 9442 Human
- Entrez Gene: 68975 Mouse
- Entrez Gene: 100174637 Orangutan
- Entrez Gene: 414430 Pig
- Entrez Gene: 496548 Xenopus tropicalis
- Entrez Gene: 393633 Zebrafish
- Omim: 605044 Human
see all -
Alternative names
- Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody
- Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody
- Cofactor required for Sp1 transcriptional activation subunit 8 antibody
see all
Images
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Western blot - Anti-MED3 antibody (ab172363)All lanes : Anti-MED3 antibody (ab172363) at 1/500 dilution
Lane 1 : MED3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 35 kDa
Protocols
Datasheets and documents
References
ab172363 has not yet been referenced specifically in any publications.
