Overview

  • Product name

  • Description

    Rabbit polyclonal to MED3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MED3 (internal sequence). The exact sequence is proprietary.
    Database link: Q6P2C8

  • Positive control

    • WB: A549 whole cell extract.
  • General notes

     This product was previously labelled as CRSP8

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 27 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 antibody
    • CRAP 34 antibody
    • CRAP34 antibody
    • CRSP 34 antibody
    • CRSP 8 antibody
    • CRSP complex subunit 8 antibody
    • CRSP34 antibody
    • CRSP8 antibody
    • MED 27 antibody
    • Med27 antibody
    • MED27_HUMAN antibody
    • Mediator complex subunit 27 antibody
    • Mediator of RNA polymerase II transcription subunit 27 antibody
    • MGC11274 antibody
    • P37 TRAP/SMCC/PC2 subunit antibody
    • Transcriptional coactivator CRSP34 antibody
    • TRAP 37 antibody
    • TRAP37 antibody
    see all

Images

  • Anti-MED3 antibody (ab229107) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

References

ab229107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229107.
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