Overview

  • Product name

    Anti-MED4 antibody [EPR7816]
    See all MED4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7816] to MED4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human MED4 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, T47D and K562 cell lysates. IHC-P: Human breast carcinoma and colon tissues. ICC/IF: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 4 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator-recruited cofactor 36 kDa component antibody
    • ARC36 antibody
    • DRIP36 antibody
    • FLJ10956 antibody
    • HSPC126 antibody
    • med4 antibody
    • MED4_HUMAN antibody
    • Mediator complex subunit 4 antibody
    • Mediator of RNA polymerase II transcription subunit 4 antibody
    • Mediator of RNA polymerase II transcription, subunit 4 homolog antibody
    • OTTHUMP00000018390 antibody
    • OTTHUMP00000018391 antibody
    • OTTHUMP00000018392 antibody
    • RP11-90M2.2 antibody
    • TRAP/SMCC/PC2 subunit p36 subunit antibody
    • TRAP36 antibody
    • VDRIP antibody
    • Vitamin D3 receptor-interacting protein complex 36 kDa component antibody
    see all

Images

  • All lanes : Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : T47D cell lysates
    Lane 3 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 30 kDa

  • ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Parrini M  et al. The C-Terminal Transactivation Domain of STAT1 Has a Gene-Specific Role in Transactivation and Cofactor Recruitment. Front Immunol 9:2879 (2018). Read more (PubMed: 30574148) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - nuclear (A549 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
35 µg
Specification
A549 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 08 2016

Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Egg)
Loading amount
25 µg
Specification
Egg
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 27 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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