RecombinantRabMAb

Recombinant Anti-MED7 antibody [EPR15410] (ab187146)

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Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Flow Cytometry - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Anti-MED7 antibody [EPR15410] (ab187146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15410] to MED7
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MED7 antibody [EPR15410]
    See all MED7 primary antibodies

  • Description

    Rabbit monoclonal [EPR15410] to MED7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MED7 aa 1-100. The exact sequence is proprietary.
    Database link: O43513

  • Positive control

    • Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.

  • General notes

     

     

    Previously labelled as CRSP9. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab187146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • Sequence similarities

    Belongs to the Mediator complex subunit 7 family.

  • Post-translational
    modifications

    Constitutively sumoylated.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activator recruited cofactor 34 kDa component antibody
      • Activator-recruited cofactor 34 kDa component antibody
      • ARC34 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
      • CRSP complex subunit 9 antibody
      • CRSP33 antibody
      • CRSP9 antibody
      • hMED7 antibody
      • MED7 antibody
      • MED7_HUMAN antibody
      • Mediator complex subunit 7 antibody
      • Mediator of RNA polymerase II transcription subunit 7 antibody
      • MGC12284 antibody
      • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
      • Transcriptional coactivator CRSP33 antibody
      see all

    Images

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : 293T lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry - Anti-MED7 antibody [EPR15410] (ab187146)
      Flow Cytometry - Anti-MED7 antibody [EPR15410] (ab187146)

      Flow Cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-MED7 antibody [EPR15410] (ab187146)
      Anti-MED7 antibody [EPR15410] (ab187146)

    References (1)

    Publishing research using ab187146? Please let us know so that we can cite the reference in this datasheet.

    ab187146 has been referenced in 1 publication.

    • Joseph C  et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). PubMed: 29588513

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