Recombinant
RabMAb

Recombinant Anti-MED7 antibody [EPR15410] (ab187146)

Submit a review Submit a question References (1)

Overview

  • Product name

    Anti-MED7 antibody [EPR15410]
    See all MED7 primary antibodies

  • Description

    Rabbit monoclonal [EPR15410] to MED7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MED7 aa 1-100. The exact sequence is proprietary.
    Database link: O43513

  • Positive control

    • Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.

  • General notes

     

     

    Previously labelled as CRSP9. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • Sequence similarities

    Belongs to the Mediator complex subunit 7 family.

  • Post-translational
    modifications

    Constitutively sumoylated.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activator recruited cofactor 34 kDa component antibody
      • Activator-recruited cofactor 34 kDa component antibody
      • ARC34 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
      • CRSP complex subunit 9 antibody
      • CRSP33 antibody
      • CRSP9 antibody
      • hMED7 antibody
      • MED7 antibody
      • MED7_HUMAN antibody
      • Mediator complex subunit 7 antibody
      • Mediator of RNA polymerase II transcription subunit 7 antibody
      • MGC12284 antibody
      • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
      • Transcriptional coactivator CRSP33 antibody
      see all

    Images

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : 293T lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry - Anti-MED7 antibody [EPR15410] (ab187146)
      Flow Cytometry - Anti-MED7 antibody [EPR15410] (ab187146)

      Flow Cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Joseph C  et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). Read more (PubMed: 29588513) »
    See 1 Publication for this product

    Publishing research using ab187146? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab187146.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com