Recombinant Anti-MED7 antibody [EPR15410] (ab187146)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15410] to MED7
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MED7 antibody [EPR15410]
See all MED7 primary antibodies -
Description
Rabbit monoclonal [EPR15410] to MED7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MED7 aa 1-100. The exact sequence is proprietary.
Database link: O43513 -
Positive control
- Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.
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General notes
Previously labelled as CRSP9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15410 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab187146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa). | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Sequence similarities
Belongs to the Mediator complex subunit 7 family. -
Post-translational
modificationsConstitutively sumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9443 Human
- Entrez Gene: 66213 Mouse
- Omim: 605045 Human
- SwissProt: O43513 Human
- SwissProt: Q9CZB6 Mouse
- Unigene: 279902 Human
- Unigene: 729173 Human
- Unigene: 24159 Mouse
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Alternative names
- Activator recruited cofactor 34 kDa component antibody
- Activator-recruited cofactor 34 kDa component antibody
- ARC34 antibody
see all
Images
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All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : 293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands. -
Flow Cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab187146 has been referenced in 1 publication.
- Joseph C et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). PubMed: 29588513