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    med7-antibody-epr15410-ab187146.pdf

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RecombinantRabMAb

Recombinant Anti-MED7 antibody [EPR15410] (ab187146)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Anti-MED7 antibody [EPR15410] (ab187146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15410] to MED7
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-MED7 antibody [EPR15410]
    See all MED7 primary antibodies
  • Description

    Rabbit monoclonal [EPR15410] to MED7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15410
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-MED7 antibody [EPR15410] - BSA and Azide free (ab250909)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human MED7 protein (ab131673)
  • Related Products

    • Recombinant Human MED7 protein (ab131673)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 7 family.
  • Post-translational
    modifications

    Constitutively sumoylated.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O43513 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9443 Human
    • Entrez Gene: 66213 Mouse
    • Omim: 605045 Human
    • SwissProt: O43513 Human
    • SwissProt: Q9CZB6 Mouse
    • Unigene: 279902 Human
    • Unigene: 729173 Human
    • Unigene: 24159 Mouse
    • Alternative names

      • Activator recruited cofactor 34 kDa component antibody
      • Activator-recruited cofactor 34 kDa component antibody
      • ARC34 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
      • CRSP complex subunit 9 antibody
      • CRSP33 antibody
      • CRSP9 antibody
      • hMED7 antibody
      • MED7 antibody
      • MED7_HUMAN antibody
      • Mediator complex subunit 7 antibody
      • Mediator of RNA polymerase II transcription subunit 7 antibody
      • MGC12284 antibody
      • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
      • Transcriptional coactivator CRSP33 antibody
      see all

    Images

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : 293T lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)
      Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)

      Intracellular flow cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-MED7 antibody [EPR15410] (ab187146)
      Anti-MED7 antibody [EPR15410] (ab187146)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab187146? Please let us know so that we can cite the reference in this datasheet.

    ab187146 has been referenced in 2 publications.

    • Schleicher EM  et al. Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. PLoS Genet 16:e1009176 (2020). PubMed: 33137164
    • Joseph C  et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). PubMed: 29588513

    Customer reviews and Q&As

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