Recombinant Anti-MED7 antibody [EPR15410] (ab187146)
![Western blot - Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-220339-anti-med7-antibody-epr15410-western-blot.jpg "Western blot - Anti-MED7 antibody [EPR15410] (ab187146)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15410] to MED7
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MED7 antibody [EPR15410]
See all MED7 primary antibodies -
Description
Rabbit monoclonal [EPR15410] to MED7 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15410 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Sequence similarities
Belongs to the Mediator complex subunit 7 family. -
Post-translational
modificationsConstitutively sumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9443 Human
- Entrez Gene: 66213 Mouse
- Omim: 605045 Human
- SwissProt: O43513 Human
- SwissProt: Q9CZB6 Mouse
- Unigene: 279902 Human
- Unigene: 729173 Human
- Unigene: 24159 Mouse
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Alternative names
- Activator recruited cofactor 34 kDa component antibody
- Activator-recruited cofactor 34 kDa component antibody
- ARC34 antibody
see all
Images
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Western blot - Anti-MED7 antibody [EPR15410] (ab187146)All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : 293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands. -
![Western blot - Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-220336-anti-med7-antibody-epr15410-western-blot.jpg)
Western blot - Anti-MED7 antibody [EPR15410] (ab187146)All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands. -
![Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-220342-anti-med7-antibody-epr15410-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)Intracellular flow cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-220341-anti-med7-antibody-epr15410-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-220340-anti-med7-antibody-epr15410-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-MED7 antibody [EPR15410] (ab187146)](https://www.abcam.com/ps/products/187/ab187146/Images/ab187146-6-benefits-of-recombinant-antibodies.png)
Anti-MED7 antibody [EPR15410] (ab187146)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab187146 has been referenced in 2 publications.
- Schleicher EM et al. Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. PLoS Genet 16:e1009176 (2020). PubMed: 33137164
- Joseph C et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). PubMed: 29588513
