Overview

  • Product name

  • Description

    Rabbit polyclonal to MED8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-288 of Human MED8 (UniProt: Q96G25-2).

  • Positive control

    • U87-MG whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab151411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the Mediator complex subunit 8 family.
  • Domain

    The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator recruited cofactor 32 kDa component antibody
    • Activator-recruited cofactor 32 kDa component antibody
    • ARC32 antibody
    • med8 antibody
    • MED8_HUMAN antibody
    • Mediator complex subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 homolog antibody
    see all

Images

  • Anti-MED8 antibody (ab151411) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 33 kDa



    12% SDS-PAGE

References

ab151411 has not yet been referenced specifically in any publications.

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