Key features and details
- Mouse polyclonal to MED9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).|
RelevanceMED9 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes.
- FLJ10193 antibody
- MED25 antibody
- MED9 antibody
All lanes : Anti-MED9 antibody (ab69323) at 1/500 dilution
Lane 1 : MED9 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 16 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
ab69323 has not yet been referenced specifically in any publications.