Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA 1/1000.

Target

  • Relevance
    MPA (also known as depo provera) is a highly potent progestational steroid that has been used worldwide as a contraceptive. There has been a concern about the possibility of an increased risk of breast cancer associated with its use. On the other hand, MPA when employed at high doses (>0.5 - 1.0g / day i.m.) can produce objective remission with improved survival in about 30% of postmenopausal women with advanced breast cancer resistant to cytotoxic drugs and endocrine therapies. Early results suggest that the response rate can be increased in patients with estrogen and / or progesterone positive receptors.
  • Alternative names
    • Medroxyprogesterone acetate antibody
    • MPA antibody

References

ab31382 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you fo ryour enquiry. I have been in contact with the originator who have kindly provided the following general cELISA testing protocol. I hope this will be helpful to you: 1. Coat the wells of an enhanced binding 96 well polystyrene microtiter plate with the Ig fraction of the antiserum, diluted in 10mM Tris, pH8.5 (125ul/well). 2. Incubate the plate for 2 hours at 37 degrees C. 3. Wash the plate 4 times with Tris buffered saline containing Tween 20 (TBST) and tap dry. 4. Prepare standard solutions of the target drug in TBST at 0 and 10ng/ml and add to the appropriate wells (50ul/well). 5. Add 75ul of conjugate (derivative of target drug conjugated to HRP), diluted in Tris buffer (pH7.2) containing EDTA, D-mannitol, sucrose, thimerosal and BSA, to each of the wells. 6. Incubate the plate for 1 hour at 25 degrees C. 7. Wash the plate 6 times over a 10 minute period with TBST. 8. Add 125ul of tetramethylbenzidine (TMB) substrate solution to each well of the plate. 9. Incubate for 15 to 20 minutes in the dark at room temperature. 10. Terminate the reaction by addition of 125ul 0.2M H2SO4 to each well. 11. Measure absorbance at 450nm using a microtiter plate reader. SHould you have any further quesitons, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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