RecombinantRabMAb

Recombinant Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free
    See all MEF2A+MEF2C primary antibodies

  • Description

    Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human MEF2C aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06413

  • Positive control

    • IHC-P: Human tonsil and colonic adenocarcinoma tissues; Mouse spleen and colon tissues; Rat spleen tissue. ICC/IF: K562 and Raji cells. Flow Cyt: Raji cells.

  • General notes

    Ab238446 is the carrier-free version of ab197070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238446 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab238446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

Images

  • Flow Cytometry - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Flow Cytometry - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Flow Cytometry analysis of Raji (human Burkitt's lymphoma) labelling MEF2A + MEF2C with purified ab197070 at 1/30 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the Human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of the colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human thymus is observed. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the mouse spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on the mouse colon epithelium, the lymphocytes on the interstitial substance showed nuclear staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the rat spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat testis is observed. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) and Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. Negative expression in Jurkat cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab238446? Please let us know so that we can cite the reference in this datasheet.

ab238446 has not yet been referenced specifically in any publications.

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