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RecombinantRabMAb

Recombinant Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

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Flow Cytometry (Intracellular) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
  • Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free
    See all MEF2A+MEF2C primary antibodies

  • Description

    Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and colonic adenocarcinoma tissues; Mouse spleen and colon tissues; Rat spleen tissue. ICC/IF: K562 and Raji cells. Flow Cyt (intra): Raji cells.

  • General notes

    ab238446 is the carrier-free version of ab197070.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Images

  • Flow Cytometry (Intracellular) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Flow Cytometry (Intracellular) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) labelling MEF2A + MEF2C with purified ab197070 at 1/30 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the Human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of the colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human thymus is observed. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the mouse spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on the mouse colon epithelium, the lymphocytes on the interstitial substance showed nuclear staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the rat spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat testis is observed. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) and Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. Negative expression in Jurkat cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197070).

  • Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
    Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab238446? Please let us know so that we can cite the reference in this datasheet.

ab238446 has not yet been referenced specifically in any publications.

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