Overview

  • Product name

    Anti-MEF2A antibody [EPR1451]
    See all MEF2A primary antibodies
  • Description

    Rabbit monoclonal [EPR1451] to MEF2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MEF2A aa 450-550. The exact sequence is proprietary.
    (Peptide available as ab151761)

  • Positive control

    • Human heart, Human skeletal muscle, HeLa and Saos-2 lysate; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.Can be blocked with MEF2A peptide (ab151761).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
    • Tissue specificity

      Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
    • Involvement in disease

      Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
    • Sequence similarities

      Belongs to the MEF2 family.
      Contains 1 MADS-box domain.
      Contains 1 Mef2-type DNA-binding domain.
    • Post-translational
      modifications

      Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
      Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
      Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
      Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADCAD1 antibody
      • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
      • MEF2 antibody
      • MEF2A antibody
      • MEF2A_HUMAN antibody
      • Myocyte enhancer factor 2A antibody
      • Myocyte-specific enhancer factor 2A antibody
      • RSRFC4 antibody
      • RSRFC9 antibody
      • Serum response factor like protein 1 antibody
      • Serum response factor-like protein 1 antibody
      see all

    Images

    • All lanes : Anti-MEF2A antibody [EPR1451] (ab109420) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • ab109420, at 1/100 dilution, staining MEF2A in HeLa cells by Immunohistochemistry.

    References

    This product has been referenced in:

    • Payne S  et al. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart. Nat Commun 10:3276 (2019). Read more (PubMed: 31332177) »
    • Carmichael RE  et al. MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1. Sci Rep 8:5263 (2018). Read more (PubMed: 29588465) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    As you have noted, fixation is often the cause of an antibody not working as expected in ICC. Abcam recommends to fix samples for ICC either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperature. If you are using acetone or methanol you may skip any further permeablization steps. We also recommend to incubate cells with 1% BSA in PBST for 30 min to block unspecific binding of the antibodies and to use the BSA/PBST solution for incubation of the primary and secondary antibodies.

    Read More

    Question
    Answer

    Thank you for your patience.
    I have now heard back from the lab:
    For ab109420, the immunogen peptide has been added to the catalog as ab151761.
    https://www.abcam.com/index.html?datasheet=151761
    For ab32866, the immunogen is not available for sale. The immunogen was a fragment of the protein made in E. coli. The bacterial expression plasmid might be available for sale, but I would need to look into that further, if you are interested in the plasmid. Please let me know.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question
    Answer

    Thank you for contacting us.
    The immunogen for ab109420 will be available for sale and be added to the catalog shortly.
    As for ab32866, I am still waiting to hear back from the lab.
    I will be in touch with more detailed information as soon as I have it.
    Thank you for your patience.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question
    Answer

    Thank you for your email.

    I would say that the band you are seeing are the right band as no other bands are on the blot and the MW is in the right range. Please keep in mind that thescale is logarithmic, not linear.

    You could use a positive control alongside your mouse heart samples, such as Human heart, Human skeletal muscle, HeLa or Saos-2 cell lysates.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for your reply and for sending the image.

    What is the MW of the two marker bands between which you obtained the protein bands?

    In general, the mouse protein has 3 isoforms (http://www.uniprot.org/uniprot/Q60929) and 3 cleavage sites which would shorten the protein if cleavage takes place. This could explain the 2 bands you are seeing.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    Could you please reply to this email and attach your WB image so I can take a look at it?

    Also, please let me know if you have any other specific questions.

    I look forward to hear back from you.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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