Key features and details
- Rabbit polyclonal to MEF2C
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MEF2C antibody
See all MEF2C primary antibodies
DescriptionRabbit polyclonal to MEF2C
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Guinea pig, Cow, Xenopus laevis, Rhesus monkey
Recombinant fragment within Human MEF2C (internal sequence). The exact sequence is proprietary.
Database link: Q06413
- WB: U-87 MG whole cell lysate. IP: K562 whole cell extract. IHC-P: Mouse forebrain tissue; Saos2 xenograft. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
FunctionTranscription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
Tissue specificityExpressed in brain and skeletal muscle.
Involvement in diseaseDefects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures.
Sequence similaritiesBelongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain.
Developmental stageExpression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
DomainThe beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
modificationsPhosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
- Information by UniProt
- C5DELq14.3 antibody
- DEL5q14.3 antibody
- MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
Anti-MEF2C antibody (ab227085) at 1/10000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
MEF2Cwas immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab227085 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227085 at 1/1000 dilution.
Lane 1: Control IgG instead of ab227085 in HeLa whole cell extract.
Lane 2: ab227085 IP in K562 whole cell extract.
Paraffin-embedded mouse forebrain tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded Saos-2 (human osteosarcoma cell line) xenograft tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MEF2C (green) using ab227085 at 1/1000 dilution in ICC/IF.
The cytoskeleton is stained with phalloidin (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227085 has not yet been referenced specifically in any publications.