Overview

  • Product name

  • Description

    Rabbit polyclonal to MEF2C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Guinea pig, Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MEF2C (internal sequence). The exact sequence is proprietary.
    Database link: Q06413

  • Positive control

    • WB: U-87 MG whole cell lysate. IP: K562 whole cell extract. IHC-P: Mouse forebrain tissue; Saos2 xenograft. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

  • Function

    Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
  • Tissue specificity

    Expressed in brain and skeletal muscle.
  • Involvement in disease

    Defects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures.
  • Sequence similarities

    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stage

    Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
  • Domain

    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications

    Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
    Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
    Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
    Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C5DELq14.3 antibody
    • DEL5q14.3 antibody
    • MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
    • MADS box transcription enhancer factor 2, polypeptide C antibody
    • MEF2C antibody
    • MEF2C_HUMAN antibody
    • Myocyte enhancer factor 2C antibody
    • Myocyte specific enhancer factor 2C antibody
    • Myocyte-specific enhancer factor 2C antibody
    • OTTHUMP00000222409 antibody
    • Similar to MADS box transcription enhancer factor 2 polypeptide C antibody
    see all

Images

  • Anti-MEF2C antibody (ab227085) at 1/10000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • MEF2Cwas immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab227085 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227085 at 1/1000 dilution.

    Lane 1: Control IgG instead of ab227085 in HeLa whole cell extract.

    Lane 2: ab227085 IP in K562 whole cell extract. 

  • Paraffin-embedded mouse forebrain tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded Saos-2 (human osteosarcoma cell line) xenograft tissue stained for MEF2C with ab227085 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MEF2C (green) using ab227085  at 1/1000 dilution in ICC/IF.

    The cytoskeleton is stained with phalloidin (red).

References

ab227085 has not yet been referenced specifically in any publications.

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