Overview

  • Product name

  • Description

    Rabbit polyclonal to MEF2D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human MEF2D aa 475-521. The exact sequence is proprietary. (NP_005911.1).
    Database link: Q14814

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab226254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • Sequence similarities

    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stage

    Present in myotubes and also in undifferentiated myoblasts.
  • Domain

    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications

    Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localization

    Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Images

  • All lanes : Anti-MEF2D antibody (ab226254) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 30 seconds
  • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for MEF2D using ab226254 at 1/200 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • MEF2D was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226254 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226254 at 1 µg/ml.

    Lane 1: ab226254 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MEF2D using ab226254 at 1/200 dilution in immunohistochemical analysis.

    Detection: DAB staining. Counterstain: Hematoxylin (blue).

References

ab226254 has not yet been referenced specifically in any publications.

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