Recombinant Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free (ab250863)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] - BSA and Azide free
See all Meis homeobox 3/MEIS3 primary antibodies -
Description
Rabbit monoclonal [EPR15416] to Meis homeobox 3/MEIS3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250863 is the carrier-free version of ab185961.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15416 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
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Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56917 Human
- Entrez Gene: 17537 Mouse
- Entrez Gene: 361514 Rat
- SwissProt: Q99687 Human
- SwissProt: P97368 Mouse
- Unigene: 380923 Human
- Unigene: 360516 Mouse
- Unigene: 480231 Mouse
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Alternative names
- Homeobox protein meis3 antibody
- Meis homeobox 3 antibody
- Meis1 myeloid ecotropic viral integration site 1 homolog 3 antibody
see all
Images
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDaThis data was developed using ab185961, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/5000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDaThis data was developed using ab185961, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Meis homeobox 3/MEIS3 antibody [EPR15416] (ab185961) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDaThis data was developed using ab185961, the same antibody clone in a different buffer formulation.
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This data was developed using ab185961, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3/MEIS3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250863 has not yet been referenced specifically in any publications.