Key features and details
- Rabbit polyclonal to MEIS1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MEIS1 antibody
See all MEIS1 primary antibodies
DescriptionRabbit polyclonal to MEIS1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human MEIS1 aa 1-90.
Database link: O00470
- WB: Jurkat, K562 and HeLa whole cell lysate (ab150035); Mouse skeletal muscle lysate. IHC-P: Human prostate cancer tissue. IP: K562 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity > 95%.
Our Abpromise guarantee covers the use of ab229962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionActs as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
Tissue specificityExpressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
Involvement in diseaseDefects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
Sequence similaritiesBelongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeo box protein Meis1 antibody
- Homeobox protein Meis1 antibody
- Leukemogenic homolog protein antibody
All lanes : Anti-MEIS1 antibody (ab229962) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse skeletal muscle lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Paraffin-embedded human prostate cancer tissue stained for MEIS1 using ab229962 at 1/100 dilution in immunohistochemical analysis.
MEIS1 was immunoprecipitated from 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 4 µg ab229962. Western blot was performed from the immunoprecipitate using ab229962. HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg Rabbit monoclonal IgG instead of ab229962 in K562 whole cell lysate.
Lane 2: ab229962 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229962 has not yet been referenced specifically in any publications.