Overview

  • Product name

  • Description

    Rabbit polyclonal to MEIS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human MEIS1 aa 1-90.
    Database link: O00470

  • Positive control

    • WB: Jurkat, K562 and HeLa whole cell lysate (ab150035); Mouse skeletal muscle lysate. IHC-P: Human prostate cancer tissue. IP: K562 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IP 1/200 - 1/2000.
IHC-P 1/20 - 1/200.

Target

  • Function

    Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
  • Tissue specificity

    Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
  • Involvement in disease

    Defects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
  • Sequence similarities

    Belongs to the TALE/MEIS homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box protein Meis1 antibody
    • Homeobox protein Meis1 antibody
    • Leukemogenic homolog protein antibody
    • MEIS 1 antibody
    • Meis homeo box 1 antibody
    • Meis homeobox 1 antibody
    • Meis1 antibody
    • Meis1 mouse homolog antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody
    • MEIS1 protein antibody
    • MEIS1_HUMAN antibody
    • MGC43380 antibody
    • Myeloid ecotropic viral integration site 1 homolog antibody
    • WUGSC:H NH0444B04.1 antibody
    see all

Images

  • All lanes : Anti-MEIS1 antibody (ab229962) at 1/2000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Mouse skeletal muscle lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human prostate cancer tissue stained for MEIS1 using ab229962 at 1/100 dilution in immunohistochemical analysis.

  • MEIS1 was immunoprecipitated from 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 4 µg ab229962. Western blot was performed from the immunoprecipitate using ab229962. HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.

    Lane 1: 1 µg Rabbit monoclonal IgG instead of ab229962 in K562 whole cell lysate.
    Lane 2: ab229962 IP in K562 whole cell lysate.
    Lane 3: K562 whole cell lysate 20 µg (Input).

References

ab229962 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
100000 cells
Treatment
Transduced with pcDNA3, Meis1-GST and Meis2-GST and harvested after 48h
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

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Verified customer

Submitted May 20 2019

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