Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MEIS1 antibody - ChIP Grade

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Dental tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Na-citrate
Permeabilization
Yes - Tween (PBS+0.3%Tween)
Specification
Dental tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Alexa568 goat anti-rabbit (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Additional Notes
Tissue was decalcified by incubating them for two weeks in 0.5M EDTA pH 7.5.

Ms. Maria Sanz Navarro

Verified customer

Submitted Aug 03 2018

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