Overview

  • Product name

  • Description

    Rabbit polyclonal to MEIS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human MEIS2 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab73163)

  • Positive control

    • This antibody gave a positive signal in human, mouse and rat brain tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab73164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163).

Target

  • Tissue specificity

    Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.
  • Sequence similarities

    Belongs to the TALE/MEIS homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeobox protein Meis2 antibody
    • HsT18361 antibody
    • Meis (mouse) homolog 2 antibody
    • Meis homeobox 2 antibody
    • Meis homolog 2 antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse) antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog 2 antibody
    • Meis1 related gene 1 antibody
    • Meis1 related protein 1 antibody
    • Meis1-related protein 1 antibody
    • MEIS2 antibody
    • MEIS2_HUMAN antibody
    • MGC2820 antibody
    • MRG1 antibody
    • TALE homeobox protein Meis2 antibody
    see all

Images

  • All lanes : Anti-MEIS2 antibody (ab73164) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Brain (Mouse) Tissue Lysate - adult normal tissue
    Lane 3 : Brain (Rat) Tissue Lysate - adult normal tissue

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab73164 in 2% Milk, overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab73164 staining human brain sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab73164 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

References

This product has been referenced in:

  • Wu YH  et al. miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2. Int J Mol Sci 16:25199-213 (2015). WB ; Human . Read more (PubMed: 26512644) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.
The Anti-MEIS2 antibody (ab73164) has been raised against a synthetic peptide taken from within the region from 450- the C-terminal of human MEIS2 (SwissProt reference O14770). The antibody should therefore detect the C-terminal end of the protein.
I hope this has sufficiently answered your question. If you require any further information please do not hesitate to contact us again.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
(agent) for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Aug 22 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms Brain Ms Heart Ms Spleen Raw264.7)
Loading amount
20 µg
Specification
Ms Brain Ms Heart Ms Spleen Raw264.7
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 8-16%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted May 25 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up