Product nameAnti-MEIS2 antibody
See all MEIS2 primary antibodies
DescriptionRabbit polyclonal to MEIS2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog
Synthetic peptide corresponding to Human MEIS2 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human, mouse and rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab73164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163).|
Tissue specificityExpressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.
Sequence similaritiesBelongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeobox protein Meis2 antibody
- HsT18361 antibody
- Meis (mouse) homolog 2 antibody
All lanes : Anti-MEIS2 antibody (ab73164) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - adult normal tissue
Lane 3 : Brain (Rat) Tissue Lysate - adult normal tissue
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab73164 in 2% Milk, overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab73164 staining human brain sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab73164 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.