Anti-MEIS2 antibody (ab73164)
Key features and details
- Rabbit polyclonal to MEIS2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MEIS2 antibody
See all MEIS2 primary antibodies -
Description
Rabbit polyclonal to MEIS2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human MEIS2 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab73163) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163). |
Target
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Tissue specificity
Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen. -
Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539573 Cow
- Entrez Gene: 612759 Dog
- Entrez Gene: 4212 Human
- Entrez Gene: 17536 Mouse
- Entrez Gene: 311311 Rat
- Omim: 601740 Human
- SwissProt: O14770 Human
- SwissProt: P97367 Mouse
see all -
Alternative names
- Homeobox protein Meis2 antibody
- HsT18361 antibody
- Meis (mouse) homolog 2 antibody
see all
Images
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All lanes : Anti-MEIS2 antibody (ab73164) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - adult normal tissue
Lane 3 : Brain (Rat) Tissue Lysate - adult normal tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab73164 in 2% Milk, overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab73164 has been referenced in 7 publications.
- Katreddi RR et al. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents. Development 149:N/A (2022). PubMed: 35781337
- Finer G et al. Stromal Transcription Factor 21 Regulates Development of the Renal Stroma via Interaction with Wnt/β-Catenin Signaling. Kidney360 3:1228-1241 (2022). PubMed: 35919523
- Lin JM et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. Elife 11:N/A (2022). PubMed: 36111787
- Yan G et al. LncRNA ILF3-AS1 promotes cell migration, invasion and EMT process in hepatocellular carcinoma via the miR-628-5p/MEIS2 axis to activate the Notch pathway. Dig Liver Dis N/A:N/A (2021). PubMed: 34053876
- Wang L et al. The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice. J Biol Chem 295:5449-5460 (2020). PubMed: 32169905
- Wan Z et al. MEIS2 promotes cell migration and invasion in colorectal cancer. Oncol Rep 42:213-223 (2019). PubMed: 31115559
- Wu YH et al. miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2. Int J Mol Sci 16:25199-213 (2015). WB ; Human . PubMed: 26512644