Recombinant
RabMAb

Recombinant Anti-MEIS2 antibody [EPR12100] (ab174270)

Overview

  • Product name

    Anti-MEIS2 antibody [EPR12100]
    See all MEIS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12100] to MEIS2
  • Host species

    Rabbit
  • Specificity

    The immunogen of ab174270 is, according to BLAST results, highly conserved (93%) with human MEIS1. According to this, the antibody is very likely to cross-react with MEIS1 but this has, to our knowledge, not been experimentally tested so far.
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MEIS2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O14770

  • Positive control

    • Fetal thymus, HeLa, HepG2 and K562 lysates. Permeabilized K562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Tissue specificity

      Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.
    • Sequence similarities

      Belongs to the TALE/MEIS homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homeobox protein Meis2 antibody
      • HsT18361 antibody
      • Meis (mouse) homolog 2 antibody
      • Meis homeobox 2 antibody
      • Meis homolog 2 antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse) antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog 2 antibody
      • Meis1 related gene 1 antibody
      • Meis1 related protein 1 antibody
      • Meis1-related protein 1 antibody
      • MEIS2 antibody
      • MEIS2_HUMAN antibody
      • MGC2820 antibody
      • MRG1 antibody
      • TALE homeobox protein Meis2 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells using ab174270 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-MEIS2 antibody [EPR12100] (ab174270) at 1/1000 dilution

      Lane 1 : Fetal thymus lysates
      Lane 2 : HeLa lysates
      Lane 3 : HepG2 lysates
      Lane 4 : K562 lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    References

    ab174270 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    Overexpression
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Sep 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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