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Synthetic peptide (Human) derived from a region of human MEK 1 + 2 that contains serine 222. The sequence is conserved in many species including mouse, rat, chimp, chicken, frog, and others.
Our Abpromise guarantee covers the use of ab4750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 43.3 kDa.|
Extracts prepared from NIH3T3 cells left untreated (lane 1) or treated (lanes 2-5) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with 0.50
ab4750 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"