Anti-MEK1 antibody (ab109556)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MEK1 antibody
See all MEK1 primary antibodies -
Description
Rabbit polyclonal to MEK1 -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Chicken, Cow, Dog, Pig
-
Immunogen
Synthetic peptide corresponding to Human MEK1 aa 353-367 (C terminal).
Sequence:KQLMVHAFIKRSDAE
Database link: Q02750 -
Positive control
- WB: Rat thymus tissue lysate, Rat skeletal muscle tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, CEM whole cell lysate, Colo320 whole cell lysate. IHC-P: Human Lung Cancer Tissue and Human Mammary Cancer Tissue ICC: MCF-7
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
-
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
Tissue specificity
Widely expressed, with extremely low levels in brain. -
Involvement in disease
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
-
Database links
- Entrez Gene: 415549 Chicken
- Entrez Gene: 533199 Cow
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 100233191 Pig
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
see all -
Alternative names
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
see all
Images
-
Western blot - Anti-MEK1 antibody (ab109556)All lanes : Anti-MEK1 antibody (ab109556) at 0.5 µg/ml
Lane 1 : Rat Skeletal Muscle Tissue Lysate
Lane 2 : Rat Kidney Tissue Lysate
Lane 3 : CEM Cell Lysate
Lane 4 : COLO20 Cell Lysate
Predicted band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody (ab109556) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue staining MEK1 using ab109556 at 1 μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Lung Cancer Tissue staining MEK1 using ab109556 at 1 μg/ml.
-
Western blot - Anti-MEK1 antibody (ab109556)All lanes : Anti-MEK1 antibody (ab109556) at 1 µg/ml
Lane 1 : Rat thymus tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat lung tissue lysate
Lane 5 : CEM whole cell lysate
Lane 6 : Colo320 whole cell lysate
Predicted band size: 43 kDa
Protocols
References
ab109556 has not yet been referenced specifically in any publications.
