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    mek1-antibody-ab109556.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Anti-MEK1 antibody (ab109556)

  • Datasheet
  • SDS
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Western blot - Anti-MEK1 antibody (ab109556)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody (ab109556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)
  • Western blot - Anti-MEK1 antibody (ab109556)

Key features and details

  • Rabbit polyclonal to MEK1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Human MAP2K1 (MEK1) knockout A-431 cell line (ab276078)

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Overview

  • Product name

    Anti-MEK1 antibody
    See all MEK1 primary antibodies
  • Description

    Rabbit polyclonal to MEK1
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human MEK1 aa 350-450 (C terminal).
    Database link: Q02750

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat thymus tissue lysate, Rat skeletal muscle tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, CEM whole cell lysate, Colo320 whole cell lysate. IHC-P: Human Lung Cancer Tissue and Human Mammary Cancer Tissue ICC: MCF-7

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human MEK1 protein (ab63209)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Rat
All applications
Mouse
Rabbit
Chicken
Cow
Dog
Pig
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Tissue specificity

    Widely expressed, with extremely low levels in brain.
  • Involvement in disease

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Target information above from: UniProt accession Q02750 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 415549 Chicken
    • Entrez Gene: 533199 Cow
    • Entrez Gene: 5604 Human
    • Entrez Gene: 26395 Mouse
    • Entrez Gene: 100233191 Pig
    • Entrez Gene: 170851 Rat
    • Omim: 176872 Human
    • SwissProt: Q02750 Human
    • SwissProt: P31938 Mouse
    • SwissProt: P29678 Rabbit
    • SwissProt: Q01986 Rat
    • Unigene: 145442 Human
    • Unigene: 248907 Mouse
    • Unigene: 5850 Rat
    see all
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase/Erk kinase 1 antibody
    • MAP2K1 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPKK 1 antibody
    • MAPKK1 antibody
    • MEK 1 antibody
    • Mek1 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • MKK 1 antibody
    • MKK1 antibody
    • MP2K1_HUMAN antibody
    • PRKMK1 antibody
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase mitogen activated, kinase 1 antibody
    • protein kinase mitogen-activated kinase 1 antibody
    see all

Images

  • Western blot - Anti-MEK1 antibody (ab109556)
    Western blot - Anti-MEK1 antibody (ab109556)
    All lanes : Anti-MEK1 antibody (ab109556) at 0.5 µg/ml

    Lane 1 : Rat Skeletal Muscle Tissue Lysate
    Lane 2 : Rat Kidney Tissue Lysate
    Lane 3 : CEM Cell Lysate
    Lane 4 : COLO20 Cell Lysate

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody (ab109556)
    Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody (ab109556)
    Immunocytochemistry/ Immunofluorescence of MCF-7 cells staining MEK1 using ab109556 at 1 μg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue staining MEK1 using ab109556 at 1 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody (ab109556)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Lung Cancer Tissue staining MEK1 using ab109556 at 1 μg/ml.

  • Western blot - Anti-MEK1 antibody (ab109556)
    Western blot - Anti-MEK1 antibody (ab109556)
    All lanes : Anti-MEK1 antibody (ab109556) at 1 µg/ml

    Lane 1 : Rat thymus tissue lysate
    Lane 2 : Rat skeletal muscle tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat lung tissue lysate
    Lane 5 : CEM whole cell lysate
    Lane 6 : Colo320 whole cell lysate

    Predicted band size: 43 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab109556? Please let us know so that we can cite the reference in this datasheet.

    ab109556 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-MAP2K1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Muscle)
    Loading amount
    50 µg
    Specification
    Muscle
    Treatment
    Different treatments
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    2.5%BSA + 2.5% Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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