Western blot abreview for Anti-MEK1 antibody [E342]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer

Secondary antibody

Dilution
1/10000

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 45 kDa Non-specific: 30 kDa

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Submitted Sep 13 2010

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