Western blot abreview for Anti-MEK1 antibody [E342]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
20 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-1% Tween

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/40000

Detection

Detection method
ECL Advance
Exposure
10 second(s)
Bands
Specific: 44 kDa Non-specific: 58 kDa
Positive control
Lane 1 = whole cell lysate
Lane 2 = cytoplasmic fraction
Lane 3 = nuclear fraction

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Submitted Jan 02 2013

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