Immunohistochemistry (Frozen sections)

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Adult heart)
Specification
Adult heart
Permeabilization
No
Fixative
Methanol

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/400

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/400

Additional data

Additional Notes
Very good antibody for immunofluorescence on frozen sections. Here the MEK1 signal is almost totally gone in the conditional MEK1 KO section, demonstrating the specificity of this antibody for MEK1.

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Submitted May 27 2013

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