Western blot abreview for Anti-MEK1 antibody [E342]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Skin)
Loading amount
15 µg
Specification
Skin
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: monoclonal anti Rabbit
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
BCIP/NBT
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
Ran 2 samples in duplicate each with different treatment and all showed bands around 45kDa

Additional data

Additional Notes
Used a PVDF membrane, Primary was incubated overnight at 4 degrees C.
Antibodies were diluted in 1.5% TBS-T

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Submitted Nov 20 2006

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