Recombinant Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E342] to MEK1 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, IHC-P, IP, WB, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Dog, Human
Overview
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Product name
Anti-MEK1 antibody [E342] - BSA and Azide free
See all MEK1 primary antibodies -
Description
Rabbit monoclonal [E342] to MEK1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-P, IP, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human -
Immunogen
Synthetic peptide within Human MEK1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q02750 -
General notes
Ab239802 is the carrier-free version of ab32091. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239802 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E342 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab239802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
Tissue specificity
Widely expressed, with extremely low levels in brain. -
Involvement in disease
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 533199 Cow
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
- SwissProt: P31938 Mouse
- SwissProt: Q01986 Rat
see all -
Alternative names
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)](https://www.abcam.com/ps/products/239/ab239802/Images/ab239802-3-ab32091261959ab32091Hela500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MEK1 with purified ab32091 at dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32091).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)](https://www.abcam.com/ps/products/239/ab239802/Images/ab239802-2-ab32091ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)Ab32091, at a 1/100 dilution, staining MEK1 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32091).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)](https://www.abcam.com/ps/products/239/ab239802/Images/ab239802-1-MEK1Primaryantibodiesab320913.jpg)
Flow Cytometry - Anti-MEK1 antibody [E342] - BSA and Azide free (ab239802)Overlay histogram showing HeLa cells stained with ab32091 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32091, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32091).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab239802 has not yet been referenced specifically in any publications.
