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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Anti-MEK1 (phospho S298) antibody (ab5613)

  • Datasheet
Submit a review Q&A (1)References (2)

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Western blot - Anti-MEK1 (phospho S298) antibody (ab5613)

    Key features and details

    • Rabbit polyclonal to MEK1 (phospho S298)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human MEK1 protein (ab63209)

    View more associated products

    Overview

    • Product name

      Anti-MEK1 (phospho S298) antibody
      See all MEK1 primary antibodies
    • Description

      Rabbit polyclonal to MEK1 (phospho S298)
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains serine 298.

    • Positive control

      • NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1. The final product is generated by affinity chromatography using a MEK 1 derived peptide that is phosphorylated at serine 298.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human MEK1 protein (ab63209)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5613 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa.
    Notes
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa.

    Target

    • Function

      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • Tissue specificity

      Widely expressed, with extremely low levels in brain.
    • Involvement in disease

      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Target information above from: UniProt accession Q02750 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 26395 Mouse
      • SwissProt: P31938 Mouse
      • Unigene: 248907 Mouse
      • Alternative names

        • Dual specificity mitogen activated protein kinase kinase 1 antibody
        • Dual specificity mitogen-activated protein kinase kinase 1 antibody
        • ERK activator kinase 1 antibody
        • MAP kinase kinase 1 antibody
        • MAP kinase/Erk kinase 1 antibody
        • MAP2K1 antibody
        • MAPK/ERK kinase 1 antibody
        • MAPKK 1 antibody
        • MAPKK1 antibody
        • MEK 1 antibody
        • Mek1 antibody
        • MEKK1 antibody
        • Mitogen activated protein kinase kinase 1 antibody
        • MKK 1 antibody
        • MKK1 antibody
        • MP2K1_HUMAN antibody
        • PRKMK1 antibody
        • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
        • Protein kinase mitogen activated, kinase 1 antibody
        • protein kinase mitogen-activated kinase 1 antibody
        see all

      Images

      • Western blot - Anti-MEK1 (phospho S298) antibody (ab5613)
        Western blot - Anti-MEK1 (phospho S298) antibody (ab5613)

        Peptide Competition: Extracts prepared from NIH3T3 cells treated with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature, following prior incubation with: the phosphopeptide immunogen (1), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphoserine containing peptide (3), or, no peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.

        Phosphatase Stripping: Extracts prepared from NIH3T3 cells not treated or treated (+PDGF) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either treated (lane 5) or not treated (lanes 6, 7) with lambda phosphatase, then incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5613 blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.



        Peptide Competition: Extracts prepared from NIH3T3 cells treated with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature, following prior incubation with: the phosphopeptide immunogen (1), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphoserine containing peptide (3), or, no peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. Phosphatase Stripping: Extracts prepared from NIH3T3 cells not treated or treated (+PDGF) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either treated (lane 5) or not treated (lanes 6, 7) with lambda phosphatase, then incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5613 blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab5613? Please let us know so that we can cite the reference in this datasheet.

      ab5613 has been referenced in 2 publications.

      • Zhu AD  et al. lncRNA-ATB promotes viability, migration, and angiogenesis in human microvascular endothelial cells by sponging microRNA-195. J Cell Biochem N/A:N/A (2019). PubMed: 30983015
      • Tanaka A  et al. Toll-like receptor 4 agonistic antibody promotes innate immunity against severe pneumonia induced by coinfection with influenza virus and Streptococcus pneumoniae. Clin Vaccine Immunol 20:977-85 (2013). PubMed: 23637040

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      can you provide immunogen sequences of the following antibodies? ab 2312 ab 5612 ab 5613 ab 4756 ab 4757 ab 9363 ab 7948 ab 4819

      Read More

      Abcam community

      Verified customer

      Asked on Jun 11 2004

      Answer

      Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS Ab9363: It is based on the C-terminal sequence: FQETARFQPGAPEAP Ab2312: The immunogen is a synthetic peptide from human Mek1. Ab5612: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains threonine 292. Ab5613: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains serine 298. Ab4756: Synthetic peptide (Human). Ab4757: Synthetic peptide (Mouse). Ab4819: Synthetic peptide (Human) derived from the region of ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187, based on the human sequence.

      Read More

      Abcam Scientific Support

      Answered on Jun 18 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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