Recombinant
RabMAb

Recombinant Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

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Overview

  • Product name

    Anti-MEK1 (phospho S298) antibody [EPR3338]
    See all MEK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3338] to MEK1 (phospho S298)

  • Host species

    Rabbit

  • Specificity

    ab96379 detects MEK1 phosphorylated at threonine 298.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IHC-Frmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 298 of Human MEK1.

  • Positive control

    • WB: HeLa cell lysates IHC-P: colonic carcinoma tissue ICC/IF: HeLa cells

  • General notes

    A trial size is available to purchase for this antibody.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab96379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
IHC-Fr 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

    • Tissue specificity

      Widely expressed, with extremely low levels in brain.

    • Involvement in disease

      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      • protein kinase mitogen-activated kinase 1 antibody
      see all

    Images

    • Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : HeLa cell lysates treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP/AP polymerized antibody

      Predicted band size: 43 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)This image is courtesy of an anonymous Abreview

      ab96379 staining MEK1 (phospho S298) in the NIH3T3  cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

      ab96379, at a 1/100 dilution, staining MEK1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379, at a 1/100 dilution, staining MEK1 in HeLa cells by Immunofluorescent analysis.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

      ab96379 showing positive staining in Thyroid gland carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

      ab96379 showing positive staining in Glioma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

      ab96379 showing positive staining in Ovarian carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
      The cells were incubated at 37øC for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt (ab120046), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120046 (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX disodium salt (ab120169), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120169 (DNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Chlorowillardiine (ab120062), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Chlorowillardiine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120062 ((S)-5-Chlorowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine (ab120063), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120063 ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (R,S)-AMPA (ab120130), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of(R,S)-AMPA, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120130 ((R,S)-AMPA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA (ab120005), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120005 ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with GYKI 52466 (ab120336), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of GYKI 52466, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120336 (GYKI 52466) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX (ab120018), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120018 (DNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)
      ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-Williardine (ab120040), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-Williardine, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120040 ((S)-Williardine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    References

    This product has been referenced in:

    • Sui H  et al. Lidocaine inhibits growth, migration and invasion of gastric carcinoma cells by up-regulation of miR-145. BMC Cancer 19:233 (2019). Read more (PubMed: 30876463) »
    • Cheng Z  et al. Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways. Mol Carcinog N/A:N/A (2019). Read more (PubMed: 30997702) »
    See all 20 Publications for this product

    Publishing research using ab96379? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HuH7 and HT29 tumor cells)
    Specification
    HuH7 and HT29 tumor cells
    Blocking step
    Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%
    Fixative
    Paraformaldehyde
    Read More

    Paul Dent

    Verified customer

    Submitted Feb 16 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 22 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HUVEK cells)
    Specification
    HUVEK cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

    Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (COS1 cell line)
    Loading amount
    25 µg
    Specification
    COS1 cell line
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

    Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse embryonic whole lung)
    Loading amount
    25 µg
    Specification
    Mouse embryonic whole lung
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

    Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    25 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

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