Name: Anti-MEK1 (phospho S298) antibody [EPR3338]
Brand: RabMAb
SKU: ab96379
Rating: 5 (6 reviews)

Product name

Anti-MEK1 (phospho S298) antibody [EPR3338]
See all MEK1 primary antibodies
Description

Rabbit monoclonal [EPR3338] to MEK1 (phospho S298)
Host species

Rabbit
Specificity

ab96379 detects MEK1 phosphorylated at threonine 298.
Tested applications

Suitable for: IHC-P, ICC/IF, WB, IHC-Frmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

A phospho specific peptide corresponding to residues surrounding serine 298 of Human MEK1.
Positive control
- WB: HeLa cell lysates IHC-P: colonic carcinoma tissue ICC/IF: HeLa cells
General notes

A trial size is available to purchase for this antibody.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3338
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab96379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100 - 1/250.
ICC/IF		1/100 - 1/250.
WB		1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
IHC-Fr		1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificity

Widely expressed, with extremely low levels in brain.
Involvement in disease

Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications

Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Target information above from: UniProt accession Q02750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
- SwissProt: P31938 Mouse
- SwissProt: Q01986 Rat
- Unigene: 145442 Human
- Unigene: 248907 Mouse
- Unigene: 5850 Rat
see all
Alternative names
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
- MAP kinase kinase 1 antibody
- MAP kinase/Erk kinase 1 antibody
- MAP2K1 antibody
- MAPK/ERK kinase 1 antibody
- MAPKK 1 antibody
- MAPKK1 antibody
- MEK 1 antibody
- Mek1 antibody
- MEKK1 antibody
- Mitogen activated protein kinase kinase 1 antibody
- MKK 1 antibody
- MKK1 antibody
- MP2K1_HUMAN antibody
- PRKMK1 antibody
- Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
- Protein kinase mitogen activated, kinase 1 antibody
see all

Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : HeLa cell lysates treated with LP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP/AP polymerized antibody

Predicted band size: 43 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)This image is courtesy of an anonymous Abreview

ab96379 staining MEK1 (phospho S298) in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379, at a 1/100 dilution, staining MEK1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379, at a 1/100 dilution, staining MEK1 in HeLa cells by Immunofluorescent analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 showing positive staining in Thyroid gland carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 showing positive staining in Glioma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 showing positive staining in Ovarian carcinoma tissue.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
The cells were incubated at 37øC for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt (ab120046), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120046 (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX disodium salt (ab120169), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX disodium salt, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120169 (DNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Chlorowillardiine (ab120062), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Chlorowillardiine, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120062 ((S)-5-Chlorowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine (ab120063), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120063 ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (R,S)-AMPA (ab120130), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of(R,S)-AMPA, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120130 ((R,S)-AMPA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA (ab120005), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120005 ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with GYKI 52466 (ab120336), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of GYKI 52466, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120336 (GYKI 52466) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX (ab120018), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120018 (DNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-Williardine (ab120040), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-Williardine, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120040 ((S)-Williardine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Sui H et al. Lidocaine inhibits growth, migration and invasion of gastric carcinoma cells by up-regulation of miR-145. BMC Cancer 19:233 (2019). Read more (PubMed: 30876463) »
Cheng Z et al. Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways. Mol Carcinog N/A:N/A (2019). Read more (PubMed: 30997702) »

See all 10 Publications for this product

Publishing research using ab96379? Please let us know so that we can cite the reference in this datasheet.

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1-6 of 6 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HuH7 and HT29 tumor cells)

Specification

HuH7 and HT29 tumor cells

Blocking step

Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%

Fixative

Paraformaldehyde

Paul Dent

Verified customer

Submitted Feb 16 2015

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (NIH3T3)

Specification

NIH3T3

Fixative

Paraformaldehyde

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 22 2013

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HUVEK cells)

Specification

HUVEK cells

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.1% Triton X100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Monkey Cell lysate - whole cell (COS1 cell line)

Loading amount

25 µg

Specification

COS1 cell line

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Mouse Tissue lysate - whole (Mouse embryonic whole lung)

Loading amount

25 µg

Specification

Mouse embryonic whole lung

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Loading amount

25 µg

Specification

HEK293

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

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Recombinant Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

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References

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Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]