Name: Anti-MEK1 (phospho S298) antibody [EPR3338]
Brand: RabMAb
SKU: ab96379
Rating: 5 (6 reviews)

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Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3338] to MEK1 (phospho S298)
Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MEK1 (phospho S298) antibody [EPR3338]
See all MEK1 primary antibodies
Description

Rabbit monoclonal [EPR3338] to MEK1 (phospho S298)
Host species

Rabbit
Specificity

ab96379 detects MEK1 phosphorylated at threonine 298.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested applications

Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

A phospho specific peptide corresponding to residues surrounding serine 298 of Human MEK1.
Positive control
- Flow Cyt: HeLa cells; ICC: HeLa cells; IHC-P: Human ovarian carcinoma tissue; WB; Rat and mouse skeletal muscle, HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3338
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250 dilution.
Flow Cyt		1/100.
ICC/IF	(3)	1/100 - 1/250.
WB	(3)	1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250 dilution.
Flow Cyt 1/100.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Application notes

Is unsuitable for IP.

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificity

Widely expressed, with extremely low levels in brain.
Involvement in disease

Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications

Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Target information above from: UniProt accession Q02750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
- SwissProt: P31938 Mouse
- SwissProt: Q01986 Rat
- Unigene: 145442 Human
- Unigene: 248907 Mouse
- Unigene: 5850 Rat
see all
Alternative names
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
- MAP kinase kinase 1 antibody
- MAP kinase/Erk kinase 1 antibody
- MAP2K1 antibody
- MAPK/ERK kinase 1 antibody
- MAPKK 1 antibody
- MAPKK1 antibody
- MEK 1 antibody
- Mek1 antibody
- MEKK1 antibody
- Mitogen activated protein kinase kinase 1 antibody
- MKK 1 antibody
- MKK1 antibody
- MP2K1_HUMAN antibody
- PRKMK1 antibody
- Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
- Protein kinase mitogen activated, kinase 1 antibody
- protein kinase mitogen-activated kinase 1 antibody
see all

Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

Lane 1 : Rat skeletal muscle lysate
Lane 2 : Rat skeletal muscle lysate, the membrane treated with phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Flow Cytometry - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEK1 with Purified ab96379 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Mouse skeletal muscle lysate, the membrane treated with phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Immunocytochemistry - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with lambda phosphatase cells labeling MEK1 with Purified ab96379 at 1/100 dilution (9.53 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100 ng/ml nocoladaze for 18 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml nocoladaze for 18 hours, then the membrane treated with phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling MEK1 with Purified ab96379 at 1/50 dilution (19.06 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab96379? Please let us know so that we can cite the reference in this datasheet.

ab96379 has been referenced in 30 publications.

Vinay P et al. Cronobacter sakazakii Infection in Early Postnatal Rats Impaired Contextual-Associated Learning: a Putative Role of C5a-Mediated NF-?ß and ASK1 Pathways. J Mol Neurosci 71:28-41 (2021). PubMed: 32567007
Yang X et al. Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell. Medicine (Baltimore) 99:e20944 (2020). PubMed: 32629699
Liao P et al. Propranolol Suppresses the Growth of Colorectal Cancer Through Simultaneously Activating Autologous CD8+ T Cells and Inhibiting Tumor AKT/MAPK Pathway. Clin Pharmacol Ther N/A:N/A (2020). PubMed: 32418204
Jia T et al. MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR. Biosci Rep 40:N/A (2020). PubMed: 32202300
Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HuH7 and HT29 tumor cells)

Specification

HuH7 and HT29 tumor cells

Blocking step

Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%

Fixative

Paraformaldehyde

Paul Dent

Verified customer

Submitted Feb 16 2015

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (NIH3T3)

Specification

NIH3T3

Fixative

Paraformaldehyde

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 22 2013

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HUVEK cells)

Specification

HUVEK cells

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.1% Triton X100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Monkey Cell lysate - whole cell (COS1 cell line)

Loading amount

25 µg

Specification

COS1 cell line

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Mouse Tissue lysate - whole (Mouse embryonic whole lung)

Loading amount

25 µg

Specification

Mouse embryonic whole lung

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Loading amount

25 µg

Specification

HEK293

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (30)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Post-translational
modifications