Western blot abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse embryonic whole lung)
Loading amount
25 µg
Specification
Mouse embryonic whole lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
4 second(s)
Bands
Specific: 45 kDa

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Submitted Nov 23 2012

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