Immunocytochemistry/ Immunofluorescence abreview for Anti-MEK1 (phospho S298) antibody [EPR3338]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HuH7 and HT29 tumor cells)
Specification
HuH7 and HT29 tumor cells
Blocking step
Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%
Fixative
Paraformaldehyde

Other product details

Incubation time
0 minute(s)
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (Alexa Fluor 594)
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/2000

Additional data

Additional Notes
Fluorescent signals were detected using a Hermes Wiscan instrument (IDEA Bio-medical, Rehovot, Israel).

Paul Dent

Verified customer

Submitted Feb 16 2015

Sign up