Product nameAnti-MEK1 (phospho T286) antibody
See all MEK1 primary antibodies
DescriptionRabbit polyclonal to MEK1 (phospho T286)
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Synthetic peptide corresponding to amino acids surrounding Threonine 286 of Human MEK1 when the Threonine residue is phosphorylated (NP_002746.1).
- HeLa whole cell lysate, treated with nocodazole.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129431 was affinity purified using an epitope specific to Phospho MEK1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 10 µg/ml.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificityWidely expressed, with extremely low levels in brain.
Involvement in diseaseDefects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/5000 (0.2µg/ml). Detection: DAB.
Detection of MEK1 by Western Blot of Immunprecipitate.
ab129431 at 1µg/ml (lanes 1-4) or Control IgG (lanes 5-6)staining MEK1 in HeLa whole cell lysate immunoprecipitated using ab129431 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 1: HeLa whole cell lysate, mock treated.
Lane 2: HeLa whole cell lysate, nocodazole treated.
Lane 3: Immunoprecipitation of MEK1 (phospho T286) from mock treated HeLa whole cell lysate.
Lane 4: Immunoprecipitation of MEK1 (phospho T286) from nocodazole treated HeLa whole cell lysate.
Lane 5: HeLa whole cell lysate, mock treated.
Lane 6: HeLa whole cell lysate, nocodazole treated.
Predicted band size : 43 kDa.
Detection: Chemiluminescence with exposure time 10 seconds.
ab129431 has not yet been referenced specifically in any publications.