Overview

  • Product name
    Anti-MEK1 (phospho T286) antibody
    See all MEK1 primary antibodies
  • Description
    Rabbit polyclonal to MEK1 (phospho T286)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to amino acids surrounding Threonine 286 of Human MEK1 when the Threonine residue is phosphorylated (NP_002746.1).

  • Positive control
    • HeLa whole cell lysate, treated with nocodazole.

Properties

Applications

Our Abpromise guarantee covers the use of ab129431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 - 10 µg/ml.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • Tissue specificity
      Widely expressed, with extremely low levels in brain.
    • Involvement in disease
      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129431 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Detection of MEK1 by Western Blot of Immunprecipitate.
      ab129431 at 1µg/ml (lanes 1-4) or Control IgG (lanes 5-6)staining MEK1 in HeLa whole cell lysate immunoprecipitated using ab129431 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 1: HeLa whole cell lysate, mock treated.
      Lane 2: HeLa whole cell lysate, nocodazole treated.
      Lane 3: Immunoprecipitation of MEK1 (phospho T286) from mock treated HeLa whole cell lysate.
      Lane 4: Immunoprecipitation of MEK1 (phospho T286) from nocodazole treated HeLa whole cell lysate.
      Lane 5: HeLa whole cell lysate, mock treated.
      Lane 6: HeLa whole cell lysate, nocodazole treated.
      Predicted band size : 43 kDa.
      Detection: Chemiluminescence with exposure time 10 seconds.

    References

    ab129431 has not yet been referenced specifically in any publications.

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