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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Anti-MEK1 (phospho T286) antibody (ab129457)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
  • Immunoprecipitation - Anti-MEK1 (phospho T286) antibody (ab129457)

Key features and details

  • Goat polyclonal to MEK1 (phospho T286)
  • Suitable for: IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant human MEK1 protein (ab63209)

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Overview

  • Product name

    Anti-MEK1 (phospho T286) antibody
    See all MEK1 primary antibodies
  • Description

    Goat polyclonal to MEK1 (phospho T286)
  • Host species

    Goat
  • Specificity

    ab129457 recognises MEK1 only when the threonine residue is phosphorylated.
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    A synthetic peptide corresponding to a region of Human MEK1 surrounding threonine 286 (NP_002746.1).

  • Positive control

    • HeLa whole cell lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human MEK1 protein (ab63209)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
IP
Human
All applications
Rat
Rabbit
Horse
Cow
Pig
Chimpanzee
Ferret
Rhesus monkey
Gorilla
Chinese hamster
Elephant
Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IP
Use at 2-10 µg/mg of lysate.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Tissue specificity

    Widely expressed, with extremely low levels in brain.
  • Involvement in disease

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Target information above from: UniProt accession Q02750 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 533199 Cow
    • Entrez Gene: 5604 Human
    • Entrez Gene: 26395 Mouse
    • Entrez Gene: 100233191 Pig
    • Entrez Gene: 170851 Rat
    • Omim: 176872 Human
    • SwissProt: Q63980 Chinese hamster
    • SwissProt: Q02750 Human
    • SwissProt: P31938 Mouse
    • SwissProt: P29678 Rabbit
    • SwissProt: Q01986 Rat
    • Unigene: 145442 Human
    • Unigene: 248907 Mouse
    • Unigene: 5850 Rat
    see all
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase/Erk kinase 1 antibody
    • MAP2K1 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPKK 1 antibody
    • MAPKK1 antibody
    • MEK 1 antibody
    • Mek1 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • MKK 1 antibody
    • MKK1 antibody
    • MP2K1_HUMAN antibody
    • PRKMK1 antibody
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase mitogen activated, kinase 1 antibody
    • protein kinase mitogen-activated kinase 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse teratoma tissue labelling MEK1 (phospho T286) with ab129457 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho T286) antibody (ab129457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MEK1 (phospho T286) with ab129457 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-MEK1 (phospho T286) antibody (ab129457)
    Immunoprecipitation - Anti-MEK1 (phospho T286) antibody (ab129457)
    ab129457, at 6µg/mg lysate (20% of IP loaded/lane), staining MEK1 in HeLa whole cell lysate mock treated (lane 1) or nocodazole treated (lane 2) by Immunoprecipitation.

    Detection: Chemiluminescence with exposure time of 30 seconds.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab129457? Please let us know so that we can cite the reference in this datasheet.

    ab129457 has been referenced in 1 publication.

    • Wang Z  et al. Polysaccharides from Enteromorpha Prolifera Ameliorate Acute Myocardial Infarction in Vitro and in Vivo via Up-Regulating HIF-1a. Int Heart J 60:964-973 (2019). PubMed: 31257333

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