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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Anti-MEK1 (phospho T292) antibody (ab5612)

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Western blot - Anti-MEK1 (phospho T292) antibody (ab5612)

    Key features and details

    • Rabbit polyclonal to MEK1 (phospho T292)
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human MEK1 protein (ab63209)

    View more associated products

    Overview

    • Product name

      Anti-MEK1 (phospho T292) antibody
      See all MEK1 primary antibodies
    • Description

      Rabbit polyclonal to MEK1 (phospho T292)
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains threonine 292.

    • Positive control

      • NIH3T3 cells +/- PDGF.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1. The final product is generated by affinity chromatography using a MEK 1 derived peptide that is phosphorylated at threonine 292.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human MEK1 protein (ab63209)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5612 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    Application Abreviews Notes
    WB
    1/1000. Predicted molecular weight: 50 kDa.
    Notes
    WB
    1/1000. Predicted molecular weight: 50 kDa.

    Target

    • Function

      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • Tissue specificity

      Widely expressed, with extremely low levels in brain.
    • Involvement in disease

      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Target information above from: UniProt accession Q02750 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      • protein kinase mitogen-activated kinase 1 antibody
      see all

    Images

    • Western blot - Anti-MEK1 (phospho T292) antibody (ab5612)
      Western blot - Anti-MEK1 (phospho T292) antibody (ab5612)
      Peptide Competition and Mutant Analysis: Recombinant wild-type (lanes 1 4) and mutant (T292A) MEK1 (lane 5) treated with ERK to induce phosphorylation was added to background extracts and resolved by SDS PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5612 antibody for two hours at room temperature, following prior incubation with: no peptide (1, 5), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5612 blocks the antibody signal, and the T292A mutant is not reactive, demonstrating the specificity of the antibody. The recombinant wild-type and mutant MEK1 were kindly provided by Dr. Natalie Ahn, University of Colorado.

      Peptide Competition and Mutant Analysis: Recombinant wild-type (lanes 1 4) and mutant (T292A) MEK1 (lane 5) treated with ERK to induce phosphorylation was added to background extracts and resolved by SDS PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5612 antibody for two hours at room temperature, following prior incubation with: no peptide (1, 5), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5612 blocks the antibody signal, and the T292A mutant is not reactive, demonstrating the specificity of the antibody. The recombinant wild-type and mutant MEK1 were kindly provided by Dr. Natalie Ahn, University of Colorado.

    Protocols

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab5612? Please let us know so that we can cite the reference in this datasheet.

    ab5612 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    can you provide immunogen sequences of the following antibodies? ab 2312 ab 5612 ab 5613 ab 4756 ab 4757 ab 9363 ab 7948 ab 4819

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2004

    Answer

    Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS Ab9363: It is based on the C-terminal sequence: FQETARFQPGAPEAP Ab2312: The immunogen is a synthetic peptide from human Mek1. Ab5612: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains threonine 292. Ab5613: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains serine 298. Ab4756: Synthetic peptide (Human). Ab4757: Synthetic peptide (Mouse). Ab4819: Synthetic peptide (Human) derived from the region of ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187, based on the human sequence.

    Read More

    Abcam Scientific Support

    Answered on Jun 18 2004

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