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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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RecombinantRabMAb

Recombinant Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)

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  • SDS
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Western blot - Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)
  • Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2365Y] to MEK1 (phospho T292)
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human MEK1 protein (ab63209)

View more associated products

Overview

  • Product name

    Anti-MEK1 (phospho T292) antibody [EPR2365Y]
    See all MEK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2365Y] to MEK1 (phospho T292)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate, treated with Nocodazole.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2365Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Alternative Versions

    • Anti-MEK1 (phospho T292) antibody [EPR2365Y] - BSA and Azide free (ab247465)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human MEK1 protein (ab63209)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/500. Predicted molecular weight: 43 kDa.
Notes
WB
1/500. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • Tissue specificity

      Widely expressed, with extremely low levels in brain.
    • Involvement in disease

      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Target information above from: UniProt accession Q02750 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5604 Human
      • Omim: 176872 Human
      • SwissProt: Q02750 Human
      • Unigene: 145442 Human
      • Alternative names

        • Dual specificity mitogen activated protein kinase kinase 1 antibody
        • Dual specificity mitogen-activated protein kinase kinase 1 antibody
        • ERK activator kinase 1 antibody
        • MAP kinase kinase 1 antibody
        • MAP kinase/Erk kinase 1 antibody
        • MAP2K1 antibody
        • MAPK/ERK kinase 1 antibody
        • MAPKK 1 antibody
        • MAPKK1 antibody
        • MEK 1 antibody
        • Mek1 antibody
        • MEKK1 antibody
        • Mitogen activated protein kinase kinase 1 antibody
        • MKK 1 antibody
        • MKK1 antibody
        • MP2K1_HUMAN antibody
        • PRKMK1 antibody
        • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
        • Protein kinase mitogen activated, kinase 1 antibody
        • protein kinase mitogen-activated kinase 1 antibody
        see all

      Images

      • Western blot - Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)
        Western blot - Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)
        All lanes : Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314) at 1/500 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HeLa cell lysate, treated with Nocodazole

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 43 kDa
        Observed band size: 43 kDa

      • Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)
        Anti-MEK1 (phospho T292) antibody [EPR2365Y] (ab76314)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab76314? Please let us know so that we can cite the reference in this datasheet.

      ab76314 has not yet been referenced specifically in any publications.

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