• Product name

    Mek1 (pS217/221) + total Mek1 ELISA Kit
    See all MEK1 kits
  • Detection method

  • Sample type

    Cell Lysate
  • Assay type

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    ab126448 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Mek1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

    This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Mek1 (Ser217/221) and total Mek1 in human, mouse and rat cell lysates (help normalize the results of phospho-Mek1 from different cell lysate being compared). An anti-pan Mek1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Mek1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Mek1 (Ser217/221) or anti-pan-Mek1 antibody is used to detect phosphorylated Mek1 (Ser217/221) or total Mek1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mek1 (Ser217/221) or total Mek1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell Lysis Buffer 1 x 5ml
    5X Assay Diluent 1 x 15ml
    Detection Antibody Mek1 1 vial
    Detection Antibody Mek1 (Ser217/221) 1 vial
    500X HRP-conjugated anti-rabbit IgG 1 x 25µl
    Mek1 Microplate (12 strips x 8 wells) coated with anti-pan Mek1 antibody 1 unit
    Positive Control: lyophilized powder from HeLa cell lysate 1 vial
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Tissue specificity

    Widely expressed, with extremely low levels in brain.
  • Involvement in disease

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 1
    • Dual specificity mitogen-activated protein kinase kinase 1
    • ERK activator kinase 1
    • MAP kinase kinase 1
    • MAP kinase/Erk kinase 1
    • MAP2K1
    • MAPK/ERK kinase 1
    • MAPKK 1
    • MAPKK1
    • MEK 1
    • Mek1
    • MEKK1
    • Mitogen activated protein kinase kinase 1
    • MKK 1
    • MKK1
    • MP2K1_HUMAN
    • PRKMK1
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1)
    • Protein kinase mitogen activated, kinase 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab126448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed by Western Blot.
  • HeLa cells were treated or untreated with TPA for 15 min. Cell lysates were analyzed using ab126448.
  • HeLa cells were treated with TPA at 37°C for 15 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of cell lysates were analyzed using ab126448.



ab126448 has not yet been referenced specifically in any publications.

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