Product nameAnti-MEK2 antibody
See all MEK2 primary antibodies
DescriptionRabbit monoclonal to MEK2
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human MEK2 aa 1-50. The exact sequence is proprietary. NP_109587.1
Database link: P36507
- ICC: KG-1 cells. IHC-P: Human prostate carcinoma and breast carcinoma tissue. WB: HepG2, HEK-293T, A549, Jurkat, RKO, U-2 OS, LNCaP, HeLa, NIH/3T3 and TCMK whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesPurified from TCS.
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab265586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44 kDa.|
|IP||Use at an assay dependent concentration.
20 µl per mg lysate.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsMAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- Cardiofaciocutaneous syndrome antibody
- CFC syndrome antibody
- CFC4 antibody
All lanes : Anti-MEK2 antibody (ab265586) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : RKO whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : LNCaP whole cell lysate
Lane 8 : HeLa whole cell lysate
Lane 9 : NIH/3T3 whole cell lysate
Lane 10 : TCMK whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 44 kDa
Exposure time: 3 minutes
Cells prepared using NETN lysis buffer.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
Immunocytochemical analysis of formalin-fixed, paraffin-embedded KG-1 cells labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
MEK2 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265586 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265586 at 1/1000 dilution.
Lane 1: ab265586 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265586 has not yet been referenced specifically in any publications.