Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MEK2 antibody [Y78] (Alexa Fluor® 647) (ab200519)

Overview

  • Product name

    Anti-MEK2 antibody [Y78] (Alexa Fluor® 647)
    See all MEK2 primary antibodies
  • Description

    Rabbit monoclonal [Y78] to MEK2 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. within Human MEK2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P36507

  • Positive control

    • ICC/IF: HeLa and wildtype HAP1 cells.
  • General notes

    Alternative versions available:
    Anti-MEK2 antibody [Y78] (ab32517) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab200519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in disease

    Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiofaciocutaneous syndrome antibody
    • CFC syndrome antibody
    • CFC4 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 2 antibody
    • map2k2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 2 antibody
    • MAPKK2 antibody
    • MEK 2 antibody
    • MEK2 antibody
    • Microtubule associated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • MKK 2 antibody
    • MKK2 antibody
    • MP2K2_HUMAN antibody
    • OTTHUMP00000165826 antibody
    • OTTHUMP00000165827 antibody
    • PRKMK 2 antibody
    • PRKMK2 antibody
    see all

Images

  • ab200519 staining MEK2 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200519 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • ab200519 staining MEK2 in wild-type HAP1 cells (top panel) and MEK2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab200519 at 1/200 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab200519 has not yet been referenced specifically in any publications.

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