Overview

  • Product name

    Anti-MEK2 (phospho T394) antibody
    See all MEK2 primary antibodies
  • Description

    Rabbit polyclonal to MEK2 (phospho T394)
  • Host species

    Rabbit
  • Specificity

    ab131095 detects endogenous levels of MEK2 only when phosphorylated at threonine 394.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding the phosphorylation site of Threonine 394 (P-G-Tp-P-T) derived from Human MEK2.

  • Positive control

    • HepG2 and HeLa cell extracts treated with UV; HeLa cells; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in disease

    Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiofaciocutaneous syndrome antibody
    • CFC syndrome antibody
    • CFC4 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 2 antibody
    • map2k2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 2 antibody
    • MAPKK2 antibody
    • MEK 2 antibody
    • MEK2 antibody
    • Microtubule associated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • MKK 2 antibody
    • MKK2 antibody
    • MP2K2_HUMAN antibody
    • OTTHUMP00000165826 antibody
    • OTTHUMP00000165827 antibody
    • PRKMK 2 antibody
    • PRKMK2 antibody
    see all

Images

  • All lanes : Anti-MEK2 (phospho T394) antibody (ab131095) at 1/500 dilution

    Lane 1 : HepG2 cell extract, untreated
    Lane 2 : HepG2 cell extract, treated with UV
    Lane 3 : HeLa cell extract, untreated
    Lane 4 : HeLa cell extract, treated with UV

    Predicted band size: 44 kDa

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK2 (phospho T394) with ab131095 antibody at 1/50 dilution. The image on the right was is treated with the synthesized peptide.
  • Immunofluorescence of methanol-fixed HeLa cells staining MEK2 (phospho T394) with ab131095 antibody at 1/100 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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