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  1. Link

    mek2-phospho-t394-antibody-ab30622.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Anti-MEK2 (phospho T394) antibody (ab30622)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (6)

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Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 (phospho T394) antibody (ab30622)
  • Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEK2 (phospho T394) antibody (ab30622)

Key features and details

  • Rabbit polyclonal to MEK2 (phospho T394)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MEK2 (phospho T394) antibody
    See all MEK2 primary antibodies
  • Description

    Rabbit polyclonal to MEK2 (phospho T394)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK2 (phospho T394).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human MEK2 protein (ab60013)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab30622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in disease

    Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Target information above from: UniProt accession P36507 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5605 Human
    • Omim: 601263 Human
    • SwissProt: P36507 Human
    • Unigene: 465627 Human
    • Alternative names

      • Cardiofaciocutaneous syndrome antibody
      • CFC syndrome antibody
      • CFC4 antibody
      • Dual specificity mitogen activated protein kinase kinase 2 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 2 antibody
      • map2k2 antibody
      • MAPK / ERK kinase 2 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 2 antibody
      • MAPKK2 antibody
      • MEK 2 antibody
      • MEK2 antibody
      • Microtubule associated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 p45 antibody
      • MKK 2 antibody
      • MKK2 antibody
      • MP2K2_HUMAN antibody
      • OTTHUMP00000165826 antibody
      • OTTHUMP00000165827 antibody
      • PRKMK 2 antibody
      • PRKMK2 antibody
      see all

    Images

    • Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
      Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
      All lanes : Anti-MEK2 (phospho T394) antibody (ab30622) at 1/500 dilution

      Lane 1 : extracts from ovary cancer cells.
      Lane 2 : extracts from ovary cancer cells, preincubated with synthesized non phosphopeptide
      Lane 3 : extracts from ovary cancer cells, preincubated
      with synthesized phosphopeptide (negative control).

      Predicted band size: 44 kDa
      Observed band size: 46 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 (phospho T394) antibody (ab30622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 (phospho T394) antibody (ab30622)

      Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue sections, using 1/50 MEK2 (Phospho Thr394) Antibody (ab30622). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

    • Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
      Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)
      Anti-MEK2 (phospho T394) antibody (ab30622) at 1/2000 dilution + VEC cell lysates

      Predicted band size: 44 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MEK2 (phospho T394) antibody (ab30622)
      Immunocytochemistry/ Immunofluorescence - Anti-MEK2 (phospho T394) antibody (ab30622)
      ICC/IF image of ab30622 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab30622? Please let us know so that we can cite the reference in this datasheet.

    ab30622 has been referenced in 6 publications.

    • Wu Q  et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011
    • Huguet A  et al. Differences in Toxic Response Induced by Three Variants of the Diarrheic Shellfish Poisoning Phycotoxins in Human Intestinal Epithelial Caco-2 Cells. Toxins (Basel) 12:N/A (2020). PubMed: 33302514
    • Huang LQ  et al. Immune Negative Regulator TIPE2 Inhibits Cervical Squamous Cancer Progression Through Erk1/2 Signaling. Open Life Sci 14:528-536 (2019). PubMed: 33817189
    • Gryshkova V  et al. Phosphoprotein expression profiles in rat kidney injury: Source for potential mechanistic biomarkers. J Cell Mol Med 23:2251-2255 (2019). PubMed: 30636369
    • Peng S  et al. Phosphodiesterase 4 Inhibitor Roflumilast Protects Rat Hippocampal Neurons from Sevoflurane Induced Injury via Modulation of MEK/ERK Signaling Pathway. Cell Physiol Biochem 45:2329-2337 (2018). PubMed: 29550808
    • Fan AC  et al. Nanofluidic proteomic assay for serial analysis of oncoprotein activation in clinical specimens. Nat Med 15:566-71 (2009). PubMed: 19363496

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-MEK2 (phospho T394) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2020

    Western blot abreview for Anti-MEK2 (phospho T394) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    colon cancer
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 13 2020

    Question

    I'm interested to know which reference papers indicates the negative feedback role of phospho-MEK2 at Thr394?? Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Aug 13 2007

    Answer

    Thank you for your enquiry. Due to my limited access to journals, I was not able to determine which specific publication indicates the negative feedback. However, I have included below all the references that we have on this product. I hope one of these publications will contain the information that you are looking for. 1) Crews C M, et al. (1992) Science. 258:478-480. 2) Alessi D R, et al. (1994) EMBO J. 13:1610-1619. 3) Rosen L B, et al. (1994) Neuron. 12:1207-1221. If you need anything else, please do not hesitate to contact me. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Aug 13 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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