Overview

  • Product name
  • Description
    Rabbit polyclonal to MEK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    MESPASSQPASMPQSK

    conjugated to KLH, corresponding to N terminal amino acids 1-16 of Human MKK3

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).

Target

  • Function
    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificity
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in disease
    Note=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Images

  • ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL). ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL).  Secondary antibody - anti-rabbit HRP

  • ab5428 staining MEK3 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab5428 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount
10 µg
Specification
brain tissue lysate, cytosolic fraction
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Dr. Maryna Polyakova

Verified customer

Submitted May 09 2012

Answer

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these additional two antibodies:
ab13898
ab28669
I hope this will be helpful. I look forward to receiving your confirmation that you would like to go ahead, and I will be pleased to arrange and send the discount codes to you.
For the remaining antibodies, I can suggest you may like to consider submitting an Abreview anyway, for which you can obtain Abpoints:
ab85612
ab75874
ab61113
ab47504
ab16476
ab85608
ab31232
ab41172
ab23832
Thank you for your time and continued interest in our products. I look forward to hearing from you.

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Answer

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application or species that it has not been previously tested in, we are able to provide our testing discount program.

In this case, the antibodiess you have listed have all been tested in western blot already, but we would be able to provide this offer to you to test the following antibodies in mouse and rat:

Discount to detect in mouse and rat (the following antibodies have not yet been tested in mouse or rat):
CDC42 Binding Protein Kinase Beta antibody ab61328
CNKR2 antibody ab64844
NEK7 antibody ab80948

Discount to test in mouse (the following antibodies have not yet been tested in mouse):
MEK3 antibody ab5428
BMX antibody ab73887
Muscarinic Acetylcholine Receptor 1 antibody ab75178

Discount to test in rat (the following antibodies have not been tested in rat):
PPP4C antibody sb70888

The following are already tested in western blot, and in mouse and/or rat as per your list. I am sorry we are therefore not able to provide a testing discount for these.
ROCK2 antibody ab85612 Already tested WB, ms and rat
CAMKIV antibody [EP2564Y] ab75874 Already tested WB, ms and rat
FAK antibody ab61113 Already tested WB, ms and rat
S6K antibody ab47504 Previously tested WB, ms and rat
PPP1A antibody ab 16476 Previously tested WB, ms and rat
5HT7 Receptor antibody ab13898 WB and mouse already tested.
NMDAR1 antibody ab28669 Previously tested WB, ms and rat
Dopamine Receptor D1 antibody ab85608 Previously tested WB, ms and rat
Glutamate Receptor 1 (AMPA subtype) antibody ab31232 Previously tested WB and rat
Nicotinic Acetylcholine Receptor alpha 4 antibody ab41172 Previously tested WB and mouse
Nicotinic Acetylcholine Receptor alpha 7 antibody ab23832 Previously tested WB and rat

For the antibodies we can provide a discount for, I can offer a discount off a future purchase if you buy the antibody(ies) now, test them in mouse, rat or both as indicated above, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount provided for each individual antibody would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test these antibodies in mouse, rat or both. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in mouse, rat or both.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN] ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Also, we are able to provide a discount for any order of 5 items or more. Therefore, once you have decided which antibodies you are going to purchase, please contact our customer service department before you place the order with a full list of the antibodies you wish to buy (orders@abcam.com). They will then be able to provide you with a quote.

I hope this will be helpful to you. I look forward to receiving confirmation that you would like to receive the discount codes. If you have any further questions, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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