Product nameAnti-MEK3 antibody [EP555Y]
See all MEK3 primary antibodies
DescriptionRabbit monoclonal [EP555Y] to MEK3
SpecificityThis antibody is specific for MEK3.
Tested applicationsSuitable for: IP, WB, Flow Cytmore details
Unsuitable for: ICC or IHC
Species reactivityReacts with: Human
Synthetic peptide within Human MEK3 aa 1-100 (N terminal). The exact sequence is proprietary.
- Jurkat, Hela, MCF-7 and K562 cell lysate.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab40836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
ab40836 has not yet been referenced specifically in any publications.