Recombinant Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
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Overview
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Product name
Anti-MEK3 antibody [EP555Y] - BSA and Azide free
See all MEK3 primary antibodies -
Description
Rabbit monoclonal [EP555Y] to MEK3 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific for MEK3.
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Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MEK3 aa 1-100 (N terminal). The exact sequence is proprietary.
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General notes
ab247295 is the carrier-free version of ab40836 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP555Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). | |
| Flow Cyt | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Involvement in disease
Note=Defects in MAP2K3 may be involved in colon cancer. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 5606 Human
- Omim: 602315 Human
- SwissProt: P46734 Human
- Unigene: 514012 Human
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Alternative names
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
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Datasheets and documents
References
ab247295 has not yet been referenced specifically in any publications.
