Recombinant Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
![Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)](https://www.abcam.com/ps/products/247/ab247295/Images/ab247295-403229-anti-mek3-antibody-ep555y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP555Y] to MEK3 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MEK3 antibody [EP555Y] - BSA and Azide free
See all MEK3 primary antibodies -
Description
Rabbit monoclonal [EP555Y] to MEK3 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific for MEK3.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MEK3 aa 1-100 (N terminal). The exact sequence is proprietary.
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General notes
ab247295 is the carrier-free version of ab40836 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP555Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Involvement in disease
Note=Defects in MAP2K3 may be involved in colon cancer. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 5606 Human
- Omim: 602315 Human
- SwissProt: P46734 Human
- Unigene: 514012 Human
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Alternative names
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
see all
Images
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Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
This data was developed using ab40836, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)](https://www.abcam.com/ps/products/247/ab247295/Images/ab247295-397879-anti-mek3-antibody-ep555y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab40836, the same antibody clone in a different buffer formulation.
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![Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)](https://www.abcam.com/ps/products/247/ab247295/Images/ab247295-3-benefits-of-recombinant-antibodies.png)
Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247295 has not yet been referenced specifically in any publications.
