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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)

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  • Certificate of Compliance
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Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
  • Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
  • Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP555Y] to MEK3 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Recombinant Human MEK3 protein (ab105578)

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Overview

  • Product name

    Anti-MEK3 antibody [EP555Y] - BSA and Azide free
    See all MEK3 primary antibodies
  • Description

    Rabbit monoclonal [EP555Y] to MEK3 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for MEK3.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MEK3 aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    ab247295 is the carrier-free version of ab40836 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP555Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-MEK3 antibody [EP555Y] (ab40836)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human MEK3 protein (ab105578)
  • Related Products

    • MCF7 whole cell lysate (ab29537)

Applications

Our Abpromise guarantee covers the use of ab247295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function

      Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
    • Tissue specificity

      Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • Involvement in disease

      Note=Defects in MAP2K3 may be involved in colon cancer.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
      Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Target information above from: UniProt accession P46734 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5606 Human
      • Omim: 602315 Human
      • SwissProt: P46734 Human
      • Unigene: 514012 Human
      • Alternative names

        • AW212142 antibody
        • dual specificity mitogen activated protein kinase kinase 3 antibody
        • Dual specificity mitogen-activated protein kinase kinase 3 antibody
        • MAP kinase kinase 3 antibody
        • map2k3 antibody
        • MAPK ERK kinase 3 antibody
        • MAPK/ERK kinase 3 antibody
        • MAPKK 3 antibody
        • MAPKK3 antibody
        • MEK 3 antibody
        • MEK3 antibody
        • Mitogen activated protein kinase kinase 3 antibody
        • MKK 3 antibody
        • MKK3 antibody
        • mMKK3b antibody
        • MP2K3_HUMAN antibody
        • PRKMK 3 antibody
        • PRKMK3 antibody
        • protein kinase, mitogen-activated, kinase 3 antibody
        • SAPK kinase 2 antibody
        • SAPKK 2 antibody
        • SAPKK2 antibody
        • Stress activated protein kinase kinase 2 antibody
        see all

      Images

      • Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
        Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)

        This data was developed using ab40836, the same antibody clone in a different buffer formulation.

        Lane 1: Wild-type HAP1 cell lysate (20 µg)

        Lane 2: MEK3knockout HAP1 cell lysate (20 µg)

        Lane 3: HeLa cell lysate (20 µg)

        Lane 4: Jurkat cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
        Western blot - Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
        All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Hela cell lysate
        Lane 3 : MCF-7 cell lysate
        Lane 4 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 39 kDa
        Observed band size: 40 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab40836, the same antibody clone in a different buffer formulation.

         

      • Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)
        Anti-MEK3 antibody [EP555Y] - BSA and Azide free (ab247295)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247295? Please let us know so that we can cite the reference in this datasheet.

      ab247295 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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