Key features and details
- Rabbit polyclonal to MEK3 (phospho S189)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-MEK3 (phospho S189) antibody
See all MEK3 primary antibodies
DescriptionRabbit polyclonal to MEK3 (phospho S189)
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- WB: HeLa cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131283 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
All lanes : Anti-MEK3 (phospho S189) antibody (ab131283) at 1/500 dilution
Lane 1 : HeLa cell extract untreated
Lane 2 : HeLa cell extract treated with PMA
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MEK3 (phospho S189) with ab131283 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunofluorescence analysis of methanol-fixed HeLa cells labelling MEK3 (phospho S189) with ab131283 at 1/100 dilution.
ab131283 has been referenced in 1 publication.
- Bassi R et al. Redox-dependent dimerization of p38a mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3. J Biol Chem 292:16161-16173 (2017). PubMed: 28739872