Overview

  • Product name

    Anti-MEK3 (phospho S189) antibody
    See all MEK3 primary antibodies
  • Description

    Rabbit polyclonal to MEK3 (phospho S189)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MEK3 (phospho S189) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    VDS(p)VA


    Database link: NP_002747.2

  • Positive control

    • WB: HeLa cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificity

    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in disease

    Note=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Images

  • All lanes : Anti-MEK3 (phospho S189) antibody (ab131283) at 1/500 dilution

    Lane 1 : HeLa cell extract untreated
    Lane 2 : HeLa cell extract treated with PMA

    Predicted band size: 39 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MEK3 (phospho S189) with ab131283 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling MEK3 (phospho S189) with ab131283 at 1/100 dilution.

References

This product has been referenced in:

  • Bassi R  et al. Redox-dependent dimerization of p38a mitogen-activated protein kinase with mitogen-activated protein kinase kinase 3. J Biol Chem 292:16161-16173 (2017). Read more (PubMed: 28739872) »
See 1 Publication for this product

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