Overview

  • Product name

    Anti-MEK3 (phospho S189) antibody
    See all MEK3 primary antibodies
  • Description

    Rabbit polyclonal to MEK3 (phospho S189)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK3 (phospho S189). A phospho specific peptide corresponding to residues surrounding S189 of Human MEK3.
    Database link: P46734

  • Positive control

    • PMA-treated HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab194809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificity

    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in disease

    Note=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Images

  • All lanes : Anti-MEK3 (phospho S189) antibody (ab194809) at 1/500 dilution

    Lane 1 : Untreated HeLa cell extract
    Lane 2 : PMA-treated HeLa cell extract

    Predicted band size: 39 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling MEK3 (phospho S189) with ab194809 at 1/50 dilution.

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling MEK3 (phospho S189) with ab194809 at 1/100 dilution. 

References

ab194809 has not yet been referenced specifically in any publications.

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