Key features and details
- Rabbit polyclonal to MEK3 (phospho S189)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-MEK3 (phospho S189) antibody
See all MEK3 primary antibodies
DescriptionRabbit polyclonal to MEK3 (phospho S189)
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human MEK3 (phospho S189). A phospho specific peptide corresponding to residues surrounding S189 of Human MEK3.
Database link: P46734
- PMA-treated HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194809 in the following tested applications.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
All lanes : Anti-MEK3 (phospho S189) antibody (ab194809) at 1/500 dilution
Lane 1 : Untreated HeLa cell extract
Lane 2 : PMA-treated HeLa cell extract
Predicted band size: 39 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling MEK3 (phospho S189) with ab194809 at 1/50 dilution.
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling MEK3 (phospho S189) with ab194809 at 1/100 dilution.
ab194809 has not yet been referenced specifically in any publications.