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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)

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Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
  • Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)

Key features and details

  • Rabbit polyclonal to MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody
  • Description

    Rabbit polyclonal to MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211)
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with MEK 3 and MEK 6 (93% homologous). This antibody shows some cross-reactivity with MEK 4 when tested in a system with high MEK 4 expression levels. It does not react with any other MEK isoforms.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211).

  • Positive control

    • Mal-E-tagged fusion protein expressing MEK 6, left inactivated or activated by adding MEKK, Anisomycin treated A549 cell line
  • General notes

    Mitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 3. The final product is generated by affinity chromatography using a MEK 3 derived peptide that is phosphorylated at serine 189 and threonine 193.
  • Primary antibody notes

    Mitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 39.3 kDa.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 39.3 kDa.

Target

  • Alternative names

    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 3 antibody
    • MAP kinase kinase 6 antibody
    • map2k3 antibody
    • MAP2K6 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 3 antibody
    • MAPKK 6 antibody
    • MAPKK3 antibody
    • MAPKK6 antibody
    • MEK 3 antibody
    • MEK 6 antibody
    • MEK3 antibody
    • MEK6 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 3 antibody
    • MKK 6 antibody
    • MKK3 antibody
    • MKK6 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • MP2K6_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • PRKMK6 antibody
    • protein kinase antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 2 antibody
    • SAPK kinase 3 antibody
    • SAPKK 2 antibody
    • SAPKK-3 antibody
    • SAPKK2 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress activated protein kinase kinase 2 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all

Images

  • Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
    Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
    All lanes : Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759) at 1/2000 dilution

    Lane 1 : A549 whole cell extract
    Lane 2 : A549 treated for 20 minutes with 200 nM of PMA
    Lane 3 : A549 treated for 30 minutes with 25 µg/mL of Anisomycin
    Lane 4 : A549 treated for 40 minutes with UV

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Predicted band size: 39.3 kDa
    Observed band size: 39, 37 kDa why is the actual band size different from the predicted?



    Two bands ~ 37 and 39 kDa corresponding to MEK3 (pS189/pT193) + MEK6 (pS207/pT211) was observed in Anisomycin treated cell line tested

  • Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
    Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)

    Peptide Competition: Extracts prepared from background extracts with Mal-E tagged fusion protein expressing MEK 6 left inactivated (6) or with MEKK added for activation (1-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4759 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic hosphoserine-containing peptide (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to MEK3/6 [pSpT189/193]/[pSpT207/211] blocks the antibody signal, demonstrating the specificity of the antibody. Please note that the Mal-E-tagged fusion protein expressing MEK6 runs at ~81 kDa.

Protocols

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab4759? Please let us know so that we can cite the reference in this datasheet.

ab4759 has not yet been referenced specifically in any publications.

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