Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody
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DescriptionRabbit polyclonal to MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211)
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Host speciesRabbit
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SpecificityThis antibody reacts with MEK 3 and MEK 6 (93% homologous). This antibody shows some cross-reactivity with MEK 4 when tested in a system with high MEK 4 expression levels. It does not react with any other MEK isoforms.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211).
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Positive control
- Mal-E-tagged fusion protein expressing MEK 6, left inactivated or activated by adding MEKK.
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General notes
Mitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 3. The final product is generated by affinity chromatography using a MEK 3 derived peptide that is phosphorylated at serine 189 and threonine 193.
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Primary antibody notesMitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 39.3 kDa. |
Images
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Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)Peptide Competition: Extracts prepared from background extracts with Mal-E tagged fusion protein expressing MEK 6 left inactivated (6) or with MEKK added for activation (1-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4759 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic hosphoserine-containing peptide (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to MEK3/6 [pSpT189/193]/[pSpT207/211] blocks the antibody signal, demonstrating the specificity of the antibody. Please note that the Mal-E-tagged fusion protein expressing MEK6 runs at ~81 kDa.
Datasheets and documents
References
ab4759 has not yet been referenced specifically in any publications.
