Key features and details
- Rabbit polyclonal to MEK3 (phospho T222)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MEK3 (phospho T222) antibody
See all MEK3 primary antibodies
DescriptionRabbit polyclonal to MEK3 (phospho T222)
Specificityab79586 detects endogenous levels of MEK3 only when phosphorylated at threonine 222.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of threonine 222 (A-K-TP-M-D)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab79586 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
All lanes : Anti-MEK3 (phospho T222) antibody (ab79586) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated
with serum (20 %, 15 minutes)
Lane 2 : Jurkat cell extracts treated
with serum (20 %, 15 minutes) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
ab79586, at a 1/50 dilution, staining MEK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
ab79586 has not yet been referenced specifically in any publications.